Anti-SGPL1 Polyclonal Antibody for IF-P, IHC-P, ELISA - O95470

Core Product Specifications and Parameters

Biological Background: SGPL1 Function and Localization

• SGPL1 encodes sphingosine-1-phosphate lyase 1 (S1PL), a pyridoxal phosphate-dependent enzyme that catalyzes the final irreversible step in sphingolipid catabolism.
• Cleaves phosphorylated sphingoid bases, particularly sphingosine-1-phosphate (S1P), into phosphoethanolamine and long-chain aldehydes, thereby regulating intracellular S1P levels and signaling (PubMed:11018465, PubMed:14570870, PubMed:24809814). Related references: PMID:11018465, PMID:14570870, PMID:24809814
• Elevates stress-induced ceramide production and apoptosis, linking sphingolipid breakdown to programmed cell death.
• Integral membrane protein localized to the endoplasmic reticulum membrane, with its catalytic domain facing the cytosol.
• Ubiquitously expressed across tissues; protein expression confirmed in fetal and adult adrenal glands (PubMed:11018465, PubMed:28165343). Related references: PMID:11018465, PMID:28165343
• Post-translational modifications include acetylation, nitration, and phosphorylation, which may modulate enzymatic activity and stability.
• Listed as a disease variant protein; mutations in SGPL1 are associated with nephrotic syndrome type 14 (NPHS14).

Experimental Guidance and Technical Tips

• The immunogen (aa 301–400) resides in the cytoplasmic domain of SGPL1 and is conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), consider using ER-specific markers for co-localization and optimize fixation/permeabilization to access the membrane target.
• In ELISA, the antibody may be used against recombinant or native SGPL1; blocking and wash conditions should be empirically optimized to reduce background.
• As an integral membrane protein, SGPL1 extraction may require mild detergents; include appropriate controls when performing western blot or ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development with reliable raw materials for sphingolipid metabolism targets, including high-quality antibodies like this anti-SGPL1 polyclonal. Our platform streamlines procurement of validated polyclonal/monoclonal antibodies, bulk ancillary reagents, and rare target materials, bridging your workflow from early research to clinical application.