Anti-SGOL1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q5FBB7

Core Product Specifications and Parameters

Biological Background: SGOL1 Function and Localization

• Shugoshin 1 (SGOL1), also known as serologically defined breast cancer antigen NY-BR-85 or shugoshin-like 1, is encoded by the SGO1 gene and belongs to the shugoshin family. Multiple isoforms exist due to alternative splicing; isoform 3 has a specialized role in centriole cohesion.
• SGOL1 plays a central role in mitotic chromosome cohesion by preventing premature dissociation of the cohesin complex from centromeres after prophase, ensuring faithful chromosome segregation.
• It acts by protecting the STAG2 subunit of cohesin from phosphorylation at the centromere until cohesin is cleaved by separase at anaphase onset, a process facilitated by its interaction with PPP2R1A.
• Phosphorylated SGOL1 is required for proper chromosome congression and attachment of spindle microtubules to the kinetochore; it is also necessary for the kinetochore localization of PLK1 and CENPF.
• SGOL1 may contribute to the tension-sensing mechanism of the spindle assembly checkpoint by modulating PLK1 kinetochore affinity.
• Subcellularly, SGOL1 localizes to the nucleus, centromere, kinetochore, spindle pole, centrosome, and nuclear speckles.
• It is widely expressed with highest levels in testis, and is strongly overexpressed in approximately 90% of breast cancers.
• Key post-translational modifications include phosphorylation, and the protein contains a coiled-coil domain; it is annotated with keywords such as cell cycle, mitosis, centromere, phosphoprotein, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 10–100 (N-terminal region) of human SGOL1; consider this epitope location when designing detection systems or epitope-tagging experiments.
• For western blotting and immunofluorescence/immunocytochemistry, validate endogenous target expression in proliferating cell lines (e.g., HeLa) where mitotic chromosomes are actively segregating.
• Cross-reactivity with mouse and rat orthologs is predicted; titration and localization patterns should be confirmed in those species.
• In ELISA-based assays, the polyclonal nature may provide robust signal but may require careful blocking and negative controls to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, supplying high-quality antibodies, antigen pairs, and ancillary reagents to diagnostic manufacturers and research laboratories worldwide. This SGOL1 Rabbit pAb supports the development of assays in chromosome biology and oncology, leveraging the target's critical role in mitotic regulation and cancer biomarker potential. Through our one-stop platform, we offer bulk sourcing, validated polyclonal/monoclonal options, and rare-target raw-material procurement to accelerate your IVD progression from concept to clinic.