Anti-SGMS1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q86VZ5

Core Product Specifications and Parameters

Biological Background: SGMS1 Function and Localization

• SGMS1 (Sphingomyelin synthase 1) is also known as Medulla oblongata-derived protein, Protein Mob, SMS1, and TMEM23.
• It is the major sphingomyelin synthase at the Golgi apparatus, catalyzing phosphocholine transfer from phosphatidylcholine to ceramide to produce sphingomyelin and diacylglycerol. Related references: PMID:14685263 PMID:17449912
• The enzymatic reaction is reversible, with direction governed by the relative concentrations of ceramide and diacylglycerol in Golgi membranes. Related references: PMID:14685263 PMID:14976195 PMID:17449912
• SGMS1 specifically converts newly synthesized ceramide, delivered from the ER by the CERT transporter, to sphingomyelin. Related references: PMID:30242129
• It forms a heteromeric complex with glucosylceramide synthase (GCS), which increases SMS activity and reduces glucosylceramide synthesis, controlling the metabolic fate of ceramide. Related references: PMID:30242129
• Subcellularly, SGMS1 localizes to the Golgi apparatus membrane.
• SGMS1 regulates signal transduction through the production of signaling lipids (DAG and ceramide) as well as sphingomyelin, a key component of membrane rafts. Related references: PMID:14976195 PMID:17449912 PMID:17982138
• It also participates in secretory transport by modulating the DAG pool at the Golgi and downstream PRKD1 signaling. Related references: PMID:18370930 PMID:21980337
• The protein is widely expressed across tissues and undergoes alternative splicing; it is annotated as a phosphoprotein, indicating post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen used to produce this antibody covers the N-terminal region (amino acids 1-134) of human SGMS1. For optimal detection, consider using it in the indicated applications: Western blot, immunofluorescence/immunocytochemistry, and ELISA.
• Predicted cross-reactivity with human and rat SGMS1 is supported by the data; validation in other species is recommended if needed.
• When performing Western blot, expect a band around 49 kDa; minor variations may occur depending on sample preparation and post-translational modifications.
• Adjust antibody dilution and incubation conditions based on your specific experimental setup; typical starting dilutions for polyclonal antibodies can be tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-SGMS1 antibody is part of our extensive offering for sphingolipid metabolism and Golgi research. Our team can assist in sourcing rare targets and scaling up production to meet your project needs.