Anti-SGLT1 Rabbit Monoclonal Antibody for WB, ELISA - P13866

Core Product Specifications and Parameters

Biological Background: SGLT1 Function and Localization

• SGLT1, also known as sodium/glucose cotransporter 1 or solute carrier family 5 member 1 (SLC5A1), is a high‑affinity Na⁺/glucose symporter.
• Functions as an electrogenic Na⁺‑coupled sugar symporter that actively transports D‑glucose and D‑galactose with a 2:1 Na⁺ to sugar stoichiometry, driven by the transmembrane sodium gradient maintained by the Na⁺/K⁺ pump. Related references: PMID:20980548, PMID:34880492, PMID:35077764
• Plays a primary role in intestinal absorption of dietary monosaccharides; located on the apical brush‑border membrane of enterocytes, transporting glucose/galactose into cells, with basolateral exit mediated by GLUT2. Additionally acts as a glucose sensor in enteroendocrine cells, triggering incretin secretion (GCG and GIP) to regulate food intake and energy homeostasis. Related references: PMID:8563765
• Together with SGLT2, mediates glucose reabsorption in the kidney, with a nonredundant role in the S3 segment of the proximal tubules.
• Transports glucose into endometrial epithelial cells, supporting glycogen synthesis, embryo nutrition, and decidual transformation of the endometrium. Related references: PMID:28974690
• Also functions as an efficient water channel, facilitating passive water transport across the plasma membrane with conductivity comparable to aquaporins, thereby contributing to transepithelial water permeability in the small intestine.
• Subcellular localization: Apical cell membrane. Tissue expression: predominantly in intestine and endometrial cells. Related references: PMID:2490366, PMID:28974690
• Post‑translational modifications include glycosylation and phosphorylation; contains disulfide bonds; several disease‑associated variants have been reported.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide mapping to residues 323‑422 of human SGLT1 (NP_000334.1). This region lies within the protein’s extracellular/large loop domain and may offer good surface accessibility for detection.
• For Western blot (WB), SGLT1 has a predicted molecular weight of approximately 73 kDa. Consider using appropriate gel percentage and transfer conditions; validate band specificity with positive control lysates from intestinal or SGLT1‑expressing cell lines.
• For ELISA, the antibody may be suitable for detecting SGLT1 in cell lysates or recombinant protein. Optimization of coating antigen concentration and antibody dilution is recommended.
• As SGLT1 is a membrane protein, inclusion of mild detergents in lysis and assay buffers may help maintain its native conformation and epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and laboratories with a comprehensive portfolio of IVD raw materials and technical sourcing expertise. This anti‑SGLT1 antibody exemplifies our readiness to supply quality‑controlled reagents for metabolic and transport‑related research. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare‑target raw materials to streamline your assay development from concept to clinic.