Anti-SGK3 Polyclonal Antibody for WB, ELISA - Q96BR1

Core Product Specifications and Parameters

Biological Background: SGK3 Function and Localization

• SGK3 (serum/glucocorticoid-regulated kinase 3), also known as cytokine-independent survival kinase (CISK) or SGK-like (SGKL), is a serine/threonine-protein kinase belonging to the SGK family. It plays a pivotal role in regulating ion channels, membrane transporters, and cellular processes such as growth, proliferation, survival, and migration.
• The kinase up-regulates a diverse array of ion channels and transporters, including epithelial sodium channels (SCNN1A/ENAC), potassium channels (KCNA3/KV1.3, KCNQ1, KCNH2/HERG), calcium channels (TRPV5/6), chloride channels (BSND), and amino acid/glutamate transporters (SLC1A5/ASCT2, SLC1A3/EAAT1, SLC1A6/EAAT4, SLC1A7/EAAT5). It also modulates Na+/H+ exchanger SLC9A3/NHE3 and Na+/K+ ATPase activity.
• SGK3 phosphorylates downstream targets including NEDD4L and GSK3B, and regulates transcription through FLII phosphorylation, positively influencing estrogen receptor (ER) transcriptional activity. It also negatively regulates ITCH/AIP4, preventing CXCR4 lysosomal sorting.
• Subcellularly, SGK3 localizes to cytoplasmic vesicles, particularly early and recycling endosomes, consistent with its role in membrane trafficking and transporter regulation.
• Human SGK3 is broadly expressed, with highest levels in pancreas, kidney, liver, heart, and brain, and lower in lung, placenta, and skeletal muscle. Notably, its expression is higher in ER-positive breast tumors compared to ER-negative ones.
• The protein contains ATP-binding and kinase domains, and undergoes phosphorylation, as indicated by its annotation as a phosphoprotein. An alternative splicing isoform has been identified (UniProt keyword).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 1–150 of human SGK3 (NP_037389.4), targeting the N-terminal region. This region may be suitable for detecting both full-length and potential N-terminal isoforms.
• In Western blotting, a band corresponding to the calculated molecular weight of approximately 57 kDa is expected. Validation across human and mouse cell or tissue lysates is recommended to confirm reactivity.
• For ELISA applications, consider using the antibody as capture or detection reagent; optimal dilutions and conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For targets like SGK3, involved in ion channel regulation and cancer biology, we can support your development pipeline with consistent, high-quality biomaterials. Leverage CamelBio’s integrated supply chain to streamline your diagnostic assay development from concept to clinic.