Anti-SGK1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00141

Core Product Specifications and Parameters

Biological Background: SGK1 Function and Localization

• SGK1 (serum/glucocorticoid-regulated kinase 1) is a serine/threonine-protein kinase that phosphorylates a wide array of substrates to regulate ion channels, membrane transporters, enzymes, and transcription factors.
• It plays a critical role in cellular stress responses and controls processes such as cell growth, proliferation, survival, and apoptosis.
• SGK1 up-regulates numerous ion channels and transporters, including ENaC, ROMK1, CFTR, TRPV5/6, and various glutamate and amino acid transporters, thereby modulating epithelial Na⁺ retention, K⁺ elimination, and nutrient transport.
• The kinase is involved in the regulation of blood pressure, cardiac repolarization, memory consolidation, and angiogenesis through phosphorylation of targets like NEDD4L, MAPT, and CREB1.
• Subcellularly, SGK1 localizes to the cytoplasm, nucleus, endoplasmic reticulum membrane, cell membrane, and mitochondrion.
• Tissue expression is broad, with highest levels in pancreas, placenta, kidney, and lung; isoform 2 is strongly expressed in brain and pancreas.
• Post-translational modifications include phosphorylation, disulfide bonds, and ubiquitin conjugation, consistent with its roles in signaling cascades (Keywords: Phosphoprotein, Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen spans the catalytic domain and C-terminal regulatory region (aa 172-431), likely enabling recognition of both isoform 1 and 2.
• For WB analysis, a band around 49 kDa is expected; consider validating with appropriate positive controls in your sample system.
• Cross-reactivity with mouse and rat indicates conserved epitopes; optimization of antibody dilution may be necessary for these species.
• For IHC-P and IF/ICC, antigen retrieval conditions should be optimized, and proper fixation methods should be tested to maintain epitope integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials including validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. As a one-stop sourcing partner for diagnostic manufacturers and research laboratories, we support SGK1-targeted assay development with reliable reagents and technical consultation. Our capabilities also cover rare target sourcing to accelerate your research and product development pipelines.