Anti-SGCE Rabbit Polyclonal Antibody for WB, ELISA - O43556

Core Product Specifications and Parameters

Biological Background: Epsilon-sarcoglycan (SGCE) Function and Localization

• SGCE (epsilon-sarcoglycan) is a member of the sarcoglycan family of transmembrane glycoproteins, encoded by the SGCE gene, also known as ESG and DYT11.
• As part of the sarcoglycan complex within the dystrophin-glycoprotein complex (DGC), SGCE forms a critical mechanical link between the intracellular F-actin cytoskeleton and the extracellular matrix, essential for muscle fiber integrity.
• SGCE is ubiquitously expressed across tissues, reflecting its fundamental role in membrane stability.
• The protein localizes predominantly to the cell membrane and sarcolemma, but is also found in the cytoplasm, associated with the cytoskeleton, and within the Golgi apparatus and dendrites.
• Post-translational modifications include N-linked glycosylation and ubiquitin-like conjugation, which may regulate protein stability and interactions.
• Mutations in SGCE are associated with myoclonus-dystonia syndrome (DYT11), a neurological movement disorder, highlighting its role beyond muscle.
• Alternative splicing generates multiple isoforms, potentially diversifying its tissue-specific functions.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–317) targets the extracellular N-terminal region of human SGCE, which is highly conserved in mouse, enabling expected cross-reactivity; when working with non-human models, verify sequence homology for your species of interest.
• For Western blot applications, denature and reduce samples by boiling in SDS loading buffer, and expect a band at ~50 kDa; optimize primary antibody dilution (e.g., 1:500–1:2000) by titration.
• In ELISA, consider coating with recombinant SGCE protein or peptide and titrate the antibody for optimal signal-to-noise ratio; this format is suitable for high-throughput screening.
• The antibody is raised in rabbit, so compatible secondary antibodies (anti-rabbit) are required; avoid using mouse secondary antibodies if mouse tissues are being analyzed directly.
• Pre-adsorption or blocking against the immunogen may reduce non-specific background; use appropriate blocking agents (e.g., 5% non-fat milk or BSA).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio proudly presents this anti-SGCE polyclonal antibody as part of our comprehensive IVD raw material portfolio. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, we support diagnostic manufacturers and research institutes in every step from concept to clinic. Rely on our expertise in the dystrophin-glycoprotein complex and neuromuscular disorders to accelerate your IVD development.