Anti-SFXN3 Rabbit Polyclonal Antibody for WB, ELISA - Q9BWM7

Core Product Specifications and Parameters

Biological Background: SFXN3 Function and Localization

• Sideroflexin-3 (SFXN3), also designated SFX3 or SLC56A3, is a member of the sideroflexin family and functions as a mitochondrial transporter for the amino acid serine.
• SFXN3 mediates the uptake of serine into mitochondria, a critical step in the one-carbon metabolism pathway that supplies one-carbon units for nucleotide and amino acid biosynthesis. Related references: PMID:30442778
• Once internalized, mitochondrial serine is converted to glycine and formate; formate exits to the cytosol and is used to generate charged folate cofactors that serve as one-carbon donors.
• The protein is an integral multi-pass membrane protein localized to the mitochondrial membrane.
• SFXN3 is subject to post-translational acetylation and has been identified in high-throughput proteomics studies.
• Its predicted molecular weight based on amino acid sequence is approximately 35.5 kDa, consistent with the observed ~36 kDa band in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 80 amino acids of human SFXN3, a region likely facing the mitochondrial intermembrane space or cytosol; this design may help target the native protein in its membrane-embedded state.
• For Western blotting, a band around 36 kDa is expected; due to potential post-translational modifications or sample conditions, minor shifts can occur, so it is advisable to validate the antibody in the relevant cell or tissue lysate.
• When performing ELISA, standard coating and blocking protocols are recommended; the antibody’s performance should be verified against recombinant SFXN3 or cell lysates expressing the target.
• Since the listed cross-reactivity is human, confirm specificity in human-derived samples; cross-reactivity with other species or family members (e.g., SFXN1, SFXN2) is not documented and should be individually assessed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic manufacturers and research laboratories. For targets like SFXN3, involved in mitochondrial one-carbon metabolism, CamelBio can assist with sourcing rare or custom antibodies, ensuring reliable supply from development to clinical application. Whether you need a single reagent or an integrated raw-material solution, CamelBio acts as a bridge to streamline your procurement and accelerate your project timeline.