Anti-SFXN2 Rabbit Polyclonal Antibody for WB and ELISA - Q96NB2

Core Product Specifications and Parameters

Biological Background: SFXN2 Function and Localization

• SFXN2 (Sideroflexin-2) is a mitochondrial amino-acid transporter that mediates serine import into mitochondria. Related references: PMID:30442778
• It plays a critical role in mitochondrial iron homeostasis by regulating heme biosynthesis. Related references: PMID:30570704
• SFXN2 belongs to the sideroflexin family of mitochondrial transporters, which are conserved across species.
• The protein is localized to both the inner and outer mitochondrial membranes.
• Widely expressed, with highest levels in kidney, liver, and pancreas.
• Post-translational modifications include acetylation, as identified by proteomics approaches.
• SFXN2 is implicated in one-carbon metabolism and amino acid transport, linking it to cellular redox balance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 10-90 of human SFXN2, an N-terminal region upstream of the predicted transmembrane domains. This may enable detection of both full-length and N-terminal fragments.
• For Western blotting, a band at approximately 36 kDa is expected in samples expressing SFXN2. Validate the antibody in the relevant sample system, such as mitochondrial fractions from kidney, liver, or pancreas.
• For ELISA, the antibody can be used in direct or indirect assays; optimal coating concentrations and detection conditions should be determined empirically.
• The antibody cross-reacts with mouse and rat SFXN2, facilitating translational studies across species. Confirmation by alignment of the immunogen sequence with orthologs is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive selection of IVD raw materials, from validated antibody pairs to optimized polyclonal and monoclonal antibodies, supporting diagnostic manufacturers and research labs. Our anti-SFXN2 rabbit polyclonal antibody is part of our mitochondrial biology portfolio, designed to accelerate assay development for metabolism and iron homeostasis research. In addition to this product, we supply bulk ancillary reagents and provide customized sourcing for rare targets, ensuring a seamless pipeline from concept to clinic.