Anti-SFXN1 Rabbit Polyclonal Antibody for WB and ELISA - Q9H9B4

Core Product Specifications and Parameters

Biological Background: SFXN1 Function and Localization

• SFXN1 (Sideroflexin-1) is a mitochondrial inner membrane amino acid transporter primarily responsible for serine import into mitochondria.
• It is a key component of the mitochondrial one-carbon metabolism pathway: imported serine is converted to glycine and formate, which is used for cytoplasmic folate-mediated one-carbon transfer reactions, essential for nucleotide synthesis and methylation. Related references: PMID:30442778
• SFXN1 may also transport other amino acids, including alanine and cysteine. Related references: PMID:30442778
• SFXN1 localizes exclusively to the mitochondrial inner membrane.
• The protein is highly expressed in blood, liver, and kidney—tissues with elevated one-carbon metabolism activity.
• Keywords associated with SFXN1 include acetylation, amino-acid transport, one-carbon metabolism, and mitochondrial inner membrane.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 87-322) covers a region conserved across human, mouse, and rat SFXN1, supporting the observed cross-reactivity. Consider sequence alignment to predict reactivity with other species.
• For Western blotting, a band of approximately 36 kDa is expected. Ensure protein extraction methods are optimized for mitochondrial membrane proteins.
• ELISA applications may use the recombinant SFXN1 protein or relevant cell lysates; validate performance in the intended sample matrix.
• As SFXN1 is a multi-pass transmembrane protein, denaturing conditions and reducing agents may influence antibody binding; optimize sample preparation protocols accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, offering research-grade antibodies like this anti-SFXN1 polyclonal antibody to support studies on mitochondrial function and one-carbon metabolism. We provide a comprehensive range of validated antibody pairs, bulk ancillary reagents, and custom-target raw-material procurement, helping streamline your development from early research to clinical application.