Anti-SFTPD (Surfactant Protein D) Rabbit Monoclonal Antibody for WB, ELISA - P35247

Core Product Specifications and Parameters

Biological Background: SFTPD (SP-D) Function and Localization

• Pulmonary surfactant-associated protein D (SP-D), encoded by the SFTPD gene (also known as COLEC7, PSPD, SFTP4), is a collectin family member belonging to the C-type lectin superfamily.
• SP-D plays a critical role in the lung's innate immune defense by binding to inhaled microorganisms, organic antigens, and toxins through its carbohydrate recognition domain, interacting with bacterial lipopolysaccharides, oligosaccharides, and fatty acids.
• The protein modulates leukocyte function in immune responses and may participate in the reorganization or turnover of pulmonary surfactant, contributing to the maintenance of alveolar homeostasis.
• SP-D is a secreted protein localized to the extracellular space and matrix, specifically abundant in the pulmonary surface film where it interfaces with air-liquid barriers.
• Tissue expression of SFTPD is prominently detected in the lung, with additional expression noted in brain, pancreas, and mature adipocytes of adipose tissue.
• The protein contains structural features including a coiled-coil domain, collagen-like region, and disulfide bonds; it undergoes post-translational modifications such as hydroxylation, glycosylation, and S-nitrosylation, and requires calcium for its lectin activity.
• SP-D binds strongly to maltose and other alpha-glucosyl moieties, which may facilitate its recognition of microbial surface carbohydrates.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; for WB, ensure that the antibody recognizes the native full-length protein under denaturing and reducing conditions, given the predicted molecular weight of ~38 kDa (human). Validate lysates from lung tissue or relevant cell lines.
• For ELISA, consider using recombinant SP-D protein or peptide as a coating antigen, and test different antibody concentrations for optimal sensitivity. Note that SP-D is secreted, so conditioned media or bronchoalveolar lavage fluid may serve as natural sample sources.
• Cross-reactivity with mouse and rat has been indicated; when using these species, verify band sizes in WB, as the mouse and rat orthologs have similar molecular weights but may exhibit slight differences. Pilot experiments with species-matched positive controls are recommended.
• As SP-D contains calcium-dependent lectin domains, the presence of calcium in buffers might affect antibody binding in certain assay formats; consider testing with and without calcium chelators if inconsistent results occur.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. For researchers investigating pulmonary surfactant proteins and innate immunity, our catalog includes high-quality reagents like this SFTPD rabbit monoclonal antibody, backed by reliable supply and technical support. Partner with CamelBio to streamline your diagnostic development from concept to clinic.