Anti-SFTPC Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - P11686

Core Product Specifications and Parameters

Biological Background: SFTPC Function and Localization

• Surfactant protein C (SFTPC), also known as SP-C or PSP-C, is a hydrophobic pulmonary surfactant protein critical for reducing alveolar surface tension and preventing alveolar collapse.
• The SFTPC gene encodes a 21 kDa precursor that is proteolytically processed to generate the mature secreted peptide, which is a key component of pulmonary surfactant.
• SFTPC is a lipoprotein modified by palmitoylation, which enhances its lipid-binding properties and facilitates its integration into the surfactant monolayer at the air-liquid interface.
• It is primarily secreted into the extracellular surfactant layer lining the alveolar walls, where it maintains film stability during breathing cycles.
• The protein contains a disulfide bond that contributes to its structural integrity and function in surface activity.
• Alternative splicing and disease-associated variants of SFTPC are linked to heritable interstitial lung diseases, underscoring its importance in lung homeostasis.

Experimental Guidance and Technical Tips

• For Western blotting, a separation gel with 12-15% acrylamide is recommended to resolve the 21 kDa protein; consider using lung tissue lysates or surfactant-enriched fractions as positive controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P) on formalin-fixed paraffin-embedded lung tissue, antigen retrieval may be required; validate the staining pattern in normal human or mouse lung sections.
• When setting up an ELISA, titration of the antibody and use of a matched pair with a capture antibody specific to SFTPC is advisable; include recombinant SFTPC as a standard.
• Given the cross-reactivity with mouse, this antibody can be utilized in mouse models for respiratory research; however, confirm species-specific reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents, to support respiratory biomarker assay development. This anti-SFTPC antibody, targeting a key surfactant protein, facilitates research into lung function and pulmonary diseases. Whether you need bulk monoclonal antibodies, rare target reagents, or technical consultation, CamelBio serves as a reliable partner from concept to clinic.