Anti-SFTPA1 Polyclonal Antibody for WB, IHC-P, ELISA - Q8IWL2

Core Product Specifications and Parameters

Biological Background: SFTPA1 Function and Localization

• SFTPA1, also known as pulmonary surfactant-associated protein A1 or collectin-4, is a member of the collectin family of C-type lectins and is primarily produced by alveolar type II cells.
• It plays a critical role in lowering surface tension at the alveolar air-liquid interface by binding surfactant phospholipids in a calcium-dependent manner, essential for normal respiration.
• SFTPA1 is secreted into the extracellular space and forms a major component of the pulmonary surfactant film and extracellular matrix.
• It acts as an innate immune pattern recognition receptor, recognizing and opsonizing pathogens like Mycobacterium tuberculosis to promote their clearance by alveolar macrophages and dendritic cells. Related references: PMID:17158455, PMID:21203928, PMID:21123169
• SFTPA1 binds the CARDS toxin of Mycoplasma pneumoniae, serving as a host receptor; siRNA knockdown reduces toxin binding and vacuolization. Related references: PMID:15845487, PMID:25139904
• Post-translational modifications include acetylation, hydroxylation, and disulfide bond formation, with a collagen-like domain and calcium-dependent lectin activity.
• It is associated with pulmonary alveolar proteinosis and interstitial lung disease (ILD), as indicated by the disease variant keyword and aliases such as ILD1.
• The protein has a molecular weight of approximately 26 kDa and undergoes alternative splicing to produce several isoforms, including SP-A1 beta, delta, gamma, and epsilon.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to a sequence within the collagen-like domain of human SFTPA1; consider this when predicting epitope specificity and potential cross-reactivity with isoforms.
• This antibody is suitable for WB, IHC-P, and ELISA as indicated; validate in your specific sample type and experimental conditions, and include appropriate controls.
• For western blot, the predicted molecular weight is ~26 kDa; however, glycosylation and other modifications may cause a shift, so verify with relevant cell or tissue lysates.
• Cross-reactivity with mouse and rat has been reported; confirm reactivity in your species of interest using appropriate tissue samples and assay conditions.
• For immunohistochemistry, antigen retrieval conditions may need optimization due to the secreted and matrix-associated nature of the target; consider using lung tissue sections as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-SFTPA1 polyclonal antibody as part of a comprehensive portfolio of IVD raw materials for pulmonary disease and innate immunity research. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—supporting your development from concept to clinic. Contact us to streamline your procurement and accelerate your diagnostic projects.