Anti-SFRS9 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13242

Core Product Specifications and Parameters

Biological Background: SFRS9 Function and Localization

• SFRS9, also known as serine/arginine-rich splicing factor 9 or SRp30c, is a member of the SR protein family involved in pre-mRNA processing.
• It functions in both constitutive splicing and alternative splicing regulation, particularly repressing the inclusion of MAPT/Tau exon 10.
• SFRS9 is primarily localized in the nucleus, consistent with its role in RNA splicing.
• The protein is a phosphoprotein and undergoes post-translational modifications including isopeptide bond formation and ubiquitin-like conjugation, which may regulate its activity.
• Tissue expression is broad, with highest levels in heart, kidney, pancreas, and placenta, and lower levels in brain, liver, lung, and skeletal muscle.
• It contains RNA-binding domains and an arginine/serine-rich (RS) domain, characteristic of SR proteins.
• SFRS9 acts as a repressor of certain splicing events, contributing to alternative splicing diversity.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole-cell lysates from human or mouse tissues with high SFRS9 expression (e.g., heart or kidney) and validate specificity with siRNA knockdown or positive controls.
• In immunofluorescence/ICC experiments, nuclear staining is expected; ensure adequate permeabilization and counterstaining to confirm localization.
• ELISA assays can be developed using recombinant SFRS9 protein or cell lysates; optimize coating concentrations and detection antibodies for best signal-to-noise ratio.
• The antibody cross-reacts with mouse SFRS9, enabling its use in mouse model studies; verify performance in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, connecting diagnostic manufacturers and research laboratories with validated reagents like the SFRS9 Rabbit pAb for splicing and gene regulation studies. Our portfolio includes optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and sourcing for rare or emerging targets, all supported by technical consulting from concept to clinic. Whether you need routine supplies or custom solutions, CamelBio bridges the gap between your research needs and reliable, high-quality raw material procurement.