Anti-SFRP5 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q5T4F7

Core Product Specifications and Parameters

Biological Background: SFRP5 Function and Localization

• SFRP5 (Secreted frizzled-related protein 5) is a member of the soluble frizzled-related proteins (sFRPS) family, which function as modulators of Wnt signaling by directly interacting with Wnt ligands.
• It plays a role in regulating cell growth and differentiation in specific cell types, and may be involved in determining the polarity of photoreceptor cells in the retina.
• Also known as Frizzled-related protein 1b (FRP-1b) and Secreted apoptosis-related protein 3 (SARP-3).
• Contains a signal peptide and is secreted into the extracellular space; classified as a secreted protein.
• Highly expressed in the retinal pigment epithelium (RPE) and pancreas, with weak expression in heart, liver, and muscle.
• Features include a disulfide bond and its involvement in the Wnt signaling pathway, with functions in developmental processes and differentiation.

Experimental Guidance and Technical Tips

• For WB, expect a signal at approximately 36kDa (theoretical molecular weight 35.6kDa from UniProt). Validate in the relevant sample system; consider using RPE or pancreatic tissue/cells.
• For IF-P and IHC-P, optimize antigen retrieval and permeabilization conditions. SFRP5 is secreted, so signal may be observed in the extracellular matrix or pericellular regions.
• Cross-reactivity with mouse and rat (as per supplier data) allows use in common model organisms. However, always confirm sequence homology for your experiment.
• For ELISA, consider pairing with a suitable capture/detection antibody; this product can be used in ELISA as indicated, but optimal working dilutions need empirical determination.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-SFRP5 polyclonal antibody supports research in Wnt signaling, development, and retinal biology. Our technical team can assist with custom sourcing for rare targets and large-scale manufacturing needs, ensuring seamless transition from concept to clinic.