Anti-SFRP4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q6FHJ7

Core Product Specifications and Parameters

Biological Background: SFRP4 Function and Localization

• SFRP4 is a member of the secreted frizzled-related protein (sFRP) family that functions as a modulator of Wnt signaling through direct interaction with Wnt ligands, thereby regulating cell growth and differentiation.
• It plays a significant role in bone morphogenesis and may act as a regulator of adult uterine morphology and function.
• SFRP4 has been implicated in increasing apoptosis during ovulation, possibly through modulation of FZ1/FZ4/WNT4 signaling.
• The protein specifically inhibits sodium-dependent phosphate uptake, demonstrating phosphaturic effects. Related references: PMID:12952927
• The protein is secreted and contains disulfide bonds and glycosylation sites, as indicated by the keywords "Disulfide bond" and "Glycoprotein".
• Tissue-specific expression includes mesenchymal cells, with high levels in the stroma of proliferative endometrium and in cardiomyocytes; in ovarian tumors, expression increases with tumor stage and inversely correlates with CTNNB1 protein levels.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 19-346) covers the majority of the mature protein, potentially generating antibodies that recognize multiple epitopes; consider titration and optimization for each application.
• For WB, a specific band around the predicted molecular weight of 40 kDa may be observed; appropriate reducing conditions are recommended.
• Since SFRP4 is a secreted protein, IF/ICC experiments should include appropriate fixation and permeabilization steps to allow detection of both intracellular and extracellular antigen pools.
• Cross-reactivity with mouse and rat is reported; however, verify specificity in the tissue/cell type of interest before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This SFRP4 antibody supports studies in Wnt signaling, bone morphogenesis, and reproductive biology, and CamelBio can supply optimized polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents to accelerate your assay development. For rare or custom targets, our sourcing network ensures reliable access to critical raw materials.