Anti-SFRP4 Monoclonal Antibody for WB, IF/ICC, ELISA - Q6FHJ7

Core Product Specifications and Parameters

Biological Background: SFRP4 Function and Localization

• SFRP4 (Secreted frizzled-related protein 4), also known as frpHE, is a member of the secreted frizzled-related protein family that modulates Wnt signaling by directly binding to Wnt ligands.
• It regulates cell growth, differentiation, and apoptosis, playing roles in bone morphogenesis, uterine function, and ovulation via FZ1/FZ4/WNT4 signaling modulation.
• SFRP4 exhibits phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Related references: PMID:12952927
• The protein contains a signal peptide for secretion and is found in the extracellular space; it is expressed in mesenchymal cells, proliferative endometrium, cardiomyocytes, and ovarian tumors.
• In ovarian tumors, SFRP4 expression increases with tumor stage and inversely correlates with CTNNB1 (β-catenin) protein levels.
• Key structural motifs include a cysteine-rich domain (CRD) and a netrin-like domain; it is a glycoprotein with disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 201-300 of human SFRP4 (Q6FHJ7), spanning part of the netrin-like domain. Consider verifying specificity against full-length and isoform sequences.
• For Western blot, expect a band at approximately 40 kDa. Validate lysate preparation methods to ensure detection of the secreted protein.
• For IF/ICC, due to the secreted nature of SFRP4, optimize cell permeabilization and consider blocking secretion to enhance intracellular staining.
• In ELISA applications, pairing with a non-competing antibody may improve detection sensitivity; validate in the appropriate sample matrix.
• Cross-reactivity with mouse and rat suggests a conserved epitope; test in relevant species tissues for comparative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibodies for Wnt signaling targets like SFRP4. We offer scalable monoclonal antibodies, ancillary reagents, and customized rare-target sourcing to accelerate your assay development from concept to clinic.