Anti-SFRP2 Polyclonal Antibody for WB and ELISA - Q96HF1

Core Product Specifications and Parameters

Biological Background: SFRP2 Function and Localization

• SFRP2, also designated SARP-1, belongs to the secreted frizzled-related protein (sFRP) family that antagonizes Wnt signaling.
• It functions as a soluble Wnt modulator by directly binding to Wnt ligands, thereby regulating downstream pathways.
• SFRP2 is implicated in the control of cell growth and differentiation in specific cellular contexts.
• The protein contributes to developmental processes, including retinal development in the eye and myogenesis.
• SFRP2 is a secreted protein that contains a cysteine-rich domain (CRD) homologous to the Wnt-binding domain of frizzled receptors.
• Tissue expression is broad, with prominent levels in adipose tissue, small intestine, and colon, and moderate expression in heart, brain, and other organs.
• UniProt keywords associated with SFRP2 include developmental protein, differentiation, Wnt signaling pathway, secreted, and signal.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 25–295 of human SFRP2, covering the CRD and part of the netrin-like domain; consider this when interpreting isoform or homology recognition.
• For Western blot, anticipate a band near 33 kDa, though post‑translational modifications may shift the apparent mass; validate in a relevant tissue such as colon or adipose.
• Cross‑reactivity with mouse is reported, supporting potential use in murine models; confirm species‑specific reactivity with appropriate controls.
• ELISA applications may benefit from pairing with a detection antibody targeting a distinct epitope; optimize assay conditions empirically.

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CamelBio empowers diagnostic manufacturers and researchers with streamlined access to premium IVD raw materials, including high-quality antibodies like this anti-SFRP2 polyclonal antibody for Wnt pathway studies. Our comprehensive portfolio spans validated antibody pairs, optimized monoclonals and polyclonals, and bulk ancillary reagents, assuring reliable sourcing from concept to clinic. For rare targets or custom development, our expert team bridges supply gaps with tailored procurement solutions.