Anti-SFPQ Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P23246

Core Product Specifications and Parameters

Biological Background: SFPQ Function and Localization

• SFPQ (splicing factor proline- and glutamine-rich), also known as PSF, POMP100, or PPP1R140, is a multifunctional DNA- and RNA-binding protein encoded by the SFPQ gene.
• It is an essential pre-mRNA splicing factor that operates early in spliceosome assembly and promotes catalytic step II, often as a heteromer with NONO. SFPQ binds intronic polypyrimidine tracts and U5 snRNA.
• SFPQ-NONO heteromer binds DNA and functions as a transcriptional activator. Related references: PMID:25765647
• SFPQ also mediates transcriptional repression by interacting with SIN3A and recruiting histone deacetylases, and regulates circadian clock genes by inhibiting CLOCK-BMAL1 activity.
• It participates in DNA non-homologous end joining (NHEJ) repair through the SFPQ-NONO heteromer, which binds DNA ends and cooperates with the Ku complex to facilitate double-strand break repair.
• Required for the assembly of nuclear speckles. Related references: PMID:25765647
• Involved in the innate immune response by forming the HDP-RNP complex, a platform for IRF3 phosphorylation via the cGAS-STING pathway. Related references: PMID:28712728
• Localizes predominantly to nuclear speckles and the nuclear matrix, but can also shuttle to the cytoplasm under certain conditions. Post‑translational modifications include phosphorylation, acetylation, methylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 600‑707 in the C‑terminal region of human SFPQ, which is conserved across human, mouse, and rat, supporting the observed cross‑reactivity.
• For Western blot, SFPQ migrates at approximately 76 kDa. Use nuclear‑enriched lysates and include loading controls for best results.
• In IHC‑P and IF/ICC, expect a nuclear speckled staining pattern; validate with appropriate tissue or cell types.
• For IP and ELISA, optimize antibody concentration and confirm target binding in the relevant sample system.
• Always follow standard protocols and run proper negative and positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, technical services, and consulting for diagnostic developers and research institutions. With this SFPQ rabbit monoclonal antibody, investigators studying pre‑mRNA splicing, transcriptional regulation, and DNA repair can source validated reagents, including antibody pairs, bulk ancillary reagents, and rare‑target raw materials. Partner with CamelBio to streamline your supply chain from concept to clinic.