Anti-SF3B6 Polyclonal Antibody for WB and ELISA - Q9Y3B4

Core Product Specifications and Parameters

Biological Background: SF3B6 Function and Localization

• SF3B6 (pre‑mRNA branch site protein p14; also known as SAP14, SF3B14a) is a 14‑kDa subunit of the SF3B complex and a core component of the 17S U2 snRNP particle.
• It directly contacts the intron branch site adenosine, acting as the nucleophile for the first catalytic step of pre‑mRNA splicing. Related references: PMID:12234937, PMID:16432215, PMID:32494006, PMID:34822310
• SF3B6 stabilizes the U2 snRNA‑intron branch site duplex, enabling splicing of introns with weak branch‑site complementarity, and is essential for the assembly of the pre‑spliceosomal ‘A’ complex. Related references: PMID:12234937, PMID:27720643, PMID:34822310
• The protein is also a functional subunit of the minor spliceosome, mediating U12‑type intron removal. Related references: PMID:15146077, PMID:33509932
• SF3B6 localizes to the nucleus, consistent with its role in mRNA processing.
• Post‑translational modifications include acetylation, isopeptide bond formation, and ubiquitin conjugation, suggesting regulatory layers in spliceosome dynamics.

Experimental Guidance and Technical Tips

• For Western blotting, nuclear or whole‑cell extracts are suitable sample preparations due to SF3B6’s nuclear localization; the expected molecular weight is approximately 15 kDa.
• When using ELISA, the polyclonal antibody can be considered as a capture or detection reagent, with optimal dilutions determined empirically.
• The immunogen covers the full‑length human SF3B6 sequence (aa 1‑125); cross‑reactivity with human and mouse has been reported, but validation in the relevant sample system is advised if working with other species.
• As a polyclonal antibody, lot‑to‑lot consistency should be monitored; include appropriate positive and negative controls in all experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, including validated antibodies, recombinant proteins, and supporting reagents. For splicing research, we source high-quality anti-SF3B6 antibodies and ancillary U2 snRNP-related reagents to accelerate your assay development. Our supply chain covers monoclonal and polyclonal antibodies, bulk orders, and rare-target raw-material sourcing, supporting projects from concept to clinic.