Anti-SF3B4 Rabbit Polyclonal Antibody for WB and ELISA - Q15427

Core Product Specifications and Parameters

Biological Background: SF3B4 Function and Localization

• SF3B4 (Splicing factor 3B subunit 4) is also known as pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP49, and Hsh49.
• SF3B4 is a component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. Related references: PMID:10882114, PMID:12234937, PMID:27720643
• SF3B4 directly participates in early spliceosome assembly and mediates recognition of the intron branch site by promoting selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Related references: PMID:12234937, PMID:32494006
• Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex required for 'A' complex assembly, which is formed by stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Related references: PMID:12234937, PMID:27720643
• Sequence-independent binding of SF3A and SF3B subcomplexes upstream of the branch site may anchor U2 snRNP to the pre-mRNA. Related references: PMID:12234937
• SF3B4 may also be involved in the assembly of the early 'E' complex. Related references: PMID:10882114
• SF3B4 acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs. Related references: PMID:15146077, PMID:33509932
• SF3B4 localizes to the nucleus and its post-translational modifications include acetylation and phosphorylation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-200), which may enhance specificity for SF3B4 detection; consider this when designing blocking or competition experiments.
• For Western blot analysis, a band around 44 kDa is expected; validate antibody specificity using knockdown or knockout controls in the relevant sample system.
• Although this antibody is validated for WB and ELISA, other applications such as immunoprecipitation or immunofluorescence may require optimization.
• Cross-reactivity with mouse and rat indicates conserved epitopes, making this antibody potentially suitable for comparative studies across these species; appropriate controls should be included.

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