Anti-SF3B3/SAP130 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15393

Core Product Specifications and Parameters

Biological Background: SF3B3/SAP130 Function and Localization

• SF3B3 (Splicing factor 3B subunit 3, also known as SAP130, SF3b130, STAF130) is a core component of the SF3B subcomplex within the 17S U2 snRNP, essential for spliceosome assembly and pre-mRNA splicing. Related references: PMID:10490618, PMID:10882114, PMID:12234937
• It plays a critical role in early spliceosome assembly by mediating recognition of the intron branch-site adenosine, the nucleophile for the first step of splicing. Related references: PMID:12234937, PMID:32494006, PMID:34822310
• Within the 17S U2 snRNP, SF3B3 together with other SF3B subunits anchors U2 snRNP to the pre-mRNA through sequence-independent binding upstream of the branch site. Related references: PMID:12234937, PMID:27720643
• SF3B3 also functions in the minor spliceosome, facilitating splicing of U12-type introns, indicating its broad role in mRNA processing. Related references: PMID:15146077, PMID:33509932
• SF3B3 localizes to the nucleus, consistent with its role in pre-mRNA splicing.
• The protein is subject to alternative splicing and phosphorylation, as identified by proteomics and direct protein sequencing.
• SF3B3 has a molecular weight of approximately 135.6 kDa (UniProt) and is involved in both constitutive and alternative splicing pathways.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1101-1217 of human SF3B3) corresponds to a C-terminal region; the antibody may recognize full-length SF3B3 and isoforms containing this epitope.
• For Western blot, a band near 136 kDa is expected; use high molecular weight markers and validate in your specific sample system.
• Cross-reactivity with human, mouse, and rat allows multi-species studies, but always confirm performance in relevant species and tissue types.
• Suitable for IHC-P, IF/ICC, and ELISA; optimization of dilution and fixation/permeabilization conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to validated IVD raw materials, including optimized monoclonal antibodies like this anti-SF3B3/SAP130 clone for splicing and spliceosome research. Our portfolio covers bulk ancillary reagents, rare target sourcing, and technical consultation—supporting your workflow from early-stage biomarker discovery to clinical assay development.