Anti-SF3B1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75533

Core Product Specifications and Parameters

Biological Background: SF3B1 Function and Localization

• SF3B1, also known as SAP155, SF3b155, and PRPF10, is a core component of the 17S U2 snRNP complex of the major spliceosome, which catalyzes the removal of introns from pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:32494006).
  Related references: PMID:12234937, PMID:27720643, PMID:32494006
• Within the U2 snRNP, SF3B1 is part of the SF3B subcomplex that is essential for 'A' complex assembly and stable binding to the branchpoint sequence (PubMed:12234937, PubMed:36104565).
  Related references: PMID:12234937, PMID:36104565
• SF3B1 directly recognizes the branch-site adenosine, the nucleophile for the first step of splicing, ensuring accurate splice site selection (PubMed:32494006, PubMed:34822310, PubMed:36104565).
  Related references: PMID:32494006, PMID:34822310, PMID:36104565
• The SF3A and SF3B subcomplexes, together with SF3B1, anchor U2 snRNP to the pre-mRNA through sequence-independent binding upstream of the branch site (PubMed:12234937).
  Related references: PMID:12234937
• SF3B1 is also a component of the minor spliceosome, which processes the rare U12-type introns (PubMed:15146077, PubMed:33509932).
  Related references: PMID:15146077, PMID:33509932
• Together with other U2 snRNP components, SF3B1 may participate in the selective processing of microRNAs from primary transcripts, such as pri-miR-17-92 (By similarity).
• SF3B1 localizes predominantly to the nucleus and nuclear speckles, and its activity is regulated by multiple post-translational modifications, including phosphorylation, acetylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 144 amino acids of human SF3B1; therefore, the antibody primarily recognizes epitopes within this region. Consider epitope accessibility in denaturing vs. native conditions when designing experiments.
• For western blot (WB), SF3B1 runs at approximately 146 kDa; ensure adequate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence (IF/ICC), nuclear and nuclear speckle staining patterns are expected; optimize fixation and permeabilization to preserve antigenicity.
• Cross-reactivity is reported for human, mouse, and rat; verify reactivity in your specific sample system if working with other species or tissues.
• ELISA applications may require careful titration of both capture and detection antibodies when used in sandwich formats; this polyclonal antibody may be suitable as a detection reagent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting IVD manufacturers, research laboratories, and diagnostics developers with a comprehensive portfolio of raw materials and technical services. This anti-SF3B1 antibody, targeting a pivotal splicing factor, is well-suited for basic research in RNA processing, spliceosome dynamics, and disease mechanisms linked to splicing defects. As your one-stop sourcing partner, CamelBio offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and tailored raw-material procurement to accelerate your project from concept to clinic.