Anti-SF3A3 Rabbit Monoclonal Antibody for WB, ELISA - Q12874

Core Product Specifications and Parameters

Biological Background: SF3A3 Function and Localization

• SF3A3 (Splicing Factor 3A Subunit 3), also known as SF3a60, SAP61, PRP9, or PRPF9, is a 59 kDa component of the SF3A subcomplex within the U2 small nuclear ribonucleoprotein (snRNP) of the spliceosome (UniProt Q12874).

• It plays a central role in pre-mRNA splicing as part of the 17S U2 SnRNP complex, which directly participates in early spliceosome assembly by recognizing the intron branch site and promoting selection of the branch-site adenosine, the nucleophile for the first transesterification step. Related references: PMID:10882114, PMID:11533230, PMID:32494006

• SF3A3 contributes to the formation of the pre-spliceosome E complex and the pre-catalytic spliceosome A complex, and it remains associated through the formation of pre-catalytic spliceosome B complexes. Related references: PMID:10882114, PMID:11533230, PMID:29360106, PMID:30315277

• Subcellularly, SF3A3 is localized to nuclear speckles and the nucleoplasm, consistent with its role in splicing factor storage and active transcription sites.

• The protein is ubiquitously expressed across tissues and is subject to post-translational modifications including phosphorylation and acetylation, as identified by proteomics studies.

• SF3A3 contains zinc-finger domains and coordinates zinc ions, indicating metal-binding functions that may stabilize protein structure or mediate interactions within the spliceosome.

• The SF3A complex, composed of SF3A1, SF3A2, and SF3A3, is essential for U2 snRNP recruitment and stable association with pre-mRNA; deficiencies in SF3A3 disrupt splicing fidelity and can lead to aberrant transcripts.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the N-terminal region (aa 1-100) of human SF3A3. For Western blotting, a specific band near 59 kDa is expected; we recommend using nuclear extracts or whole-cell lysates from human (e.g., HeLa, HEK293) or mouse cell lines. Pre-validation of the antibody in the relevant sample system is advised.

• For ELISA applications, the peptide immunogen can be used as a coating antigen; however, detection of native SF3A3 in complex samples may require optimization. Consider pairing with a detection antibody targeting a non-overlapping epitope.

• Cross-reactivity has been reported for human and mouse; confirm reactivity in other species if required, as sequence homology varies.

• Use appropriate negative controls (e.g., siRNA knockdown) to verify specificity in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a curated portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For spliceosome targets like SF3A3, we offer flexible sourcing solutions to support assay development and biomarker research. As your one-stop bridge, we facilitate efficient procurement of rare-target reagents, enabling seamless transition from concept to clinical application.