Anti-SF3A3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q12874

Core Product Specifications and Parameters

Biological Background: SF3A3 Function and Localization

• SF3A3 is a subunit of the SF3A subcomplex within the 17S U2 small nuclear ribonucleoprotein (snRNP), a essential component of the spliceosome. Related references: PMID:10882114 PMID:11533230 PMID:32494006
• It plays a critical role in pre-mRNA splicing by mediating recognition of the intron branch site and promoting selection of the branch-point adenosine, the nucleophile for the first catalytic step. Related references: PMID:10882114 PMID:11533230 PMID:32494006
• SF3A3 contributes to the assembly of the 17S U2 snRNP and the subsequent formation of the pre-spliceosome 'E' complex and pre-catalytic spliceosome 'A' and 'B' complexes. Related references: PMID:10882114 PMID:11533230 PMID:29360106 PMID:30315277
• The protein localizes to nuclear speckles and the nucleoplasm, consistent with its role in mRNA processing.
• SF3A3 is ubiquitously expressed across tissues, reflecting the fundamental requirement for splicing in all cell types.
• It contains zinc-finger domains and binds zinc, indicative of metal-binding properties; post-translational modifications include acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 292-501 of human SF3A3 (NP_006793.1). For detection of endogenous SF3A3, consider using the antibody in western blot (expected band ~59 kDa) and immunofluorescence applications after validating in the relevant sample system.
• For IHC-P, antigen retrieval and antibody titration may be necessary; test the antibody on a panel of positive and negative control tissues to confirm specificity.
• Cross-reactivity with mouse and rat SF3A3 has been indicated; sequence homology in the immunogen region should be assessed when applying to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVF diagnostic manufacturers and research laboratories with comprehensive access to high-quality raw materials, like this SF3A3 polyclonal antibody designed for mRNA splicing studies. From validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare target sourcing, we support your entire development workflow—from concept to clinical application. Reach out to leverage our expertise and accelerate your splicing-related research projects.