Anti-SF3A1 Rabbit Polyclonal Antibody for WB, ELISA - Q15459

Core Product Specifications and Parameters

Biological Background: SF3A1 Function and Localization

• SF3A1 (Splicing factor 3A subunit 1), also known as SF3a120, SAP 114, PRP21, PRPF21, SF3A120, is a spliceosome component encoded by the SF3A1 gene.
• It functions in the 17S U2 snRNP complex, recognizing the intron branch site adenosine and promoting early spliceosome assembly. Related references: PMID:10882114 PMID:11533230 PMID:32494006
• SF3A1 also participates in pre-catalytic spliceosome 'B' complexes during pre-mRNA splicing. Related references: PMID:29360106 PMID:30315277
• It localizes to the nucleus, particularly in nuclear speckles.
• SF3A1 is ubiquitously expressed in human tissues.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation; the protein contains repeat regions.
• Alternative splicing of SF3A1 produces multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 715-785) targets a C-terminal region of SF3A1; verify epitope accessibility in your assay system.
• For Western blotting, the expected molecular weight is ~89 kDa. Validate detection in lysates from human, mouse, or rat cells and include appropriate controls.
• When using ELISA, optimize coating conditions and antibody titration to achieve the required sensitivity and specificity.
• Cross-reactivity with mouse and rat is predicted based on sequence similarity; independently confirm reactivity in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing solution for IVD raw materials, supporting spliceosome and RNA processing research with high-quality antibodies like this anti-SF3A1 polyclonal antibody. Our catalog includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and comprehensive ancillary reagents to accelerate your development pipeline. Partner with CamelBio to access a reliable supply chain tailored to your diagnostic needs.