Anti-SF1 Rabbit Polyclonal Antibody for WB and ELISA - Q15637

Core Product Specifications and Parameters

Biological Background: Splicing Factor 1 Function and Localization

• SF1 (Splicing Factor 1), also known as BBP (Branchpoint Binding Protein), mBBP, ZFM1, ZNF162, is a nuclear protein that plays a crucial role in pre-mRNA splicing.
• It is necessary for the ATP-dependent first step of spliceosome assembly by binding to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA.
• SF1 may also act as a transcription repressor, linking splicing to transcription regulation.
• The protein contains a zinc-finger domain, consistent with its classification as a zinc-finger protein and its metal-binding properties.
• Subcellularly, SF1 is localized to the nucleus, where splicing and transcription occur.
• SF1 is widely expressed, detected in tissues including lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver, and heart.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its activity in mRNA processing and other cellular processes.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 1-280 of human SF1 (NP_973727.1). This N-terminal region may be considered for epitope recognition in assays.
• For Western blotting, a band around 68 kDa is expected based on the predicted molecular weight. Validate band specificity using appropriate positive (e.g., HeLa, HEK293) and negative controls.
• The antibody is suitable for ELISA applications; consider optimizing coating antigen and antibody dilution for your specific assay format.
• Cross-reactivity is reported for human and mouse; if working with other species, verify sequence homology and perform validation in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive IVD raw material procurement. Whether you need validated antibody pairs for SF1-related splicing research, optimized monoclonal/polyclonal antibodies, or bulk ancillary reagents for assay development, our one-stop platform streamlines sourcing from concept to clinic. We also specialize in rare target raw-material sourcing to bridge gaps in your supply chain.