Anti-SET/TAF1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q01105

Core Product Specifications and Parameters

Biological Background: SET/TAF1 Function and Localization

• Protein SET (also known as PHAPII, I2PP2A, TAF-I) exists as multiple isoforms with distinct functions in apoptosis, transcription regulation, and chromatin dynamics.
• Isoform 2 exerts anti-apoptotic activity by inhibiting the granzyme A-activated DNase NME1; GZMA cleavage of SET during CTL-induced apoptosis releases NME1 inhibition.
• Both isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A (PP2A), contributing to cellular signaling modulation.
• SET isoforms inhibit EP300/CREBBP and PCAF-mediated acetylation of histones and nucleosomes, primarily targeting histone H4, leading to transcriptional silencing and preventing active DNA demethylation.
• The protein stimulates DNA replication of the adenovirus genome when complexed with viral core proteins, with isoform 2 exhibiting higher specific activity.
• Subcellular localization includes cytoplasm, cytosol, endoplasmic reticulum, and nucleus (nucleoplasm), consistent with its diverse roles.
• Widely expressed across tissues; expression is low in quiescent cells and elevated in Wilms' tumor, highlighting its association with cell proliferation and cancer.
• Post-translational modifications: Acetylation, methylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation have been reported, potentially regulating its functional interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to aa 200–290 of human SET/TAF1, a region conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blotting, expect a band around 33 kDa; additional bands may reflect isoforms or post-translational modifications. Validate with appropriate controls.
• In IHC-P and IF/ICC, consider nuclear/cytoplasmic staining patterns; optimize antigen retrieval and permeabilization protocols per your sample type.
• As a rabbit monoclonal antibody, it is suitable for IP and ELISA; perform titration experiments to determine optimal dilutions for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, providing a one-stop solution from concept to clinic. For research into apoptosis, transcriptional regulation, or chromatin biology, we offer access to validated monoclonal antibodies like this anti-SET/TAF1 clone, along with antibody pairs, bulk ancillary reagents, and rare target material sourcing. Our team provides technical consultation to help streamline your assay development and manufacturing workflows.