Anti-SETMAR Rabbit Polyclonal Antibody for IHC-P and ELISA - Q53H47

Core Product Specifications and Parameters

Biological Background: SETMAR Function and Localization

• SETMAR is a fusion protein derived from a SET domain histone methyltransferase and the transposase domain of an Hsmar1 transposon, also known as Metnase.
• It catalyzes histone H3 methylation at lysine 4 (H3K4) and lysine 36 (H3K36), and specifically mediates dimethylation of H3K36 at DNA double-strand break sites, which is critical for repair protein recruitment. Related references: PMID:16332963, PMID:21187428, PMID:22231448
• SETMAR retains sequence-specific DNA-binding activity to the 19-mer core of Hsmar1 terminal inverted repeats and possesses DNA nicking and end-joining activities. Related references: PMID:16332963, PMID:16672366, PMID:17403897
• It functions in DNA double-strand break repair through the non-homologous end-joining pathway, facilitating the restoration of genomic integrity. Related references: PMID:16332963, PMID:21187428, PMID:22231448
• Additionally, SETMAR regulates replication fork restart and DNA decatenation by stimulating topoisomerase II alpha (TOP2A) activity. Related references: PMID:18790802, PMID:20457750
• Localization is primarily nuclear, associated with chromosomes.
• Tissue expression is widespread, with highest levels in placenta and ovary and lowest in skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-300 of human SETMAR, encompassing the N-terminal region including part of the SET domain. This region may offer good accessibility for detection in IHC-P applications, but formalin fixation could mask epitopes; antigen retrieval may be necessary.
• As a polyclonal antibody, it can recognize multiple epitopes, potentially providing robust signal in ELISA and IHC-P, but batch-to-batch variability should be considered. Titration is recommended for optimal working dilution.
• Suitable for human samples only; cross-reactivity with other species has not been reported. Positive and negative controls should include relevant human tissue or cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with seamless access to high-quality IVD raw materials, including validated antibodies like the anti-SETMAR polyclonal antibody, tailored for DNA repair and chromatin biology research. Complement your projects with our extensive portfolio of bulk ancillary reagents, antigen-specific antibody pairs, and rare-target sourcing services, all supported by technical consultation from concept to clinic.