Anti-SETDB2 Rabbit Polyclonal Antibody for WB, ELISA - Q96T68

Core Product Specifications and Parameters

Biological Background: SETDB2 Function and Localization

• SETDB2 is a histone-lysine N-methyltransferase (KMT1F) that catalyzes trimethylation of histone H3 at lysine 9 (H3K9me3), a repressive epigenetic mark.
• H3K9me3 serves as a docking site for HP1 proteins (CBX1, CBX3, CBX5), leading to transcriptional silencing and heterochromatin formation.
• SETDB2 plays a role in chromosome condensation and segregation during mitosis, contributing to proper cell division.
• It is involved in left-right axis specification during early embryonic development.
• The protein localizes to the nucleus and is associated with chromosomes.
• SETDB2 is ubiquitously expressed, with highest levels in heart, testis, and ovary.
• Contains a SET domain and multiple zinc finger motifs, indicating metal-binding and methyltransferase activity.
• Alternative splicing generates multiple transcript variants.

Experimental Guidance and Technical Tips

• For Western blot, consider using nuclear extraction protocols to enrich for the target protein, as SETDB2 localizes to the nucleus.
• Validate antibody specificity in the relevant sample system (human, mouse, or rat) and include appropriate controls to confirm the expected band at ~82 kDa.
• Due to the polyclonal nature of the antibody, lot-to-lot variability may occur; include internal reference standards for reproducibility.
• The immunogen covers amino acids 148–378, a region that may influence epitope recognition; consider mapping epitopes if needed for competitive assays.
• ELISA can be employed to determine antibody titer and assess batch consistency; optimize coating conditions and detection parameters.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs seamless access to validated IVD raw materials, including polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This anti-SETDB2 antibody supports epigenetic and cell cycle research needs, and our team can assist with scaling supply or sourcing complementary antibody pairs for assay development. From concept to clinic, we are your dedicated partner for building robust diagnostic solutions.