Anti-SETDB1 Rabbit Polyclonal Antibody (KO Validated) for WB, IF-P, ELISA - Q15047

Core Product Specifications and Parameters

Biological Background: SETDB1 Function and Localization

• SETDB1, also known as ESET or KMT1E, is a histone-lysine N-methyltransferase that specifically trimethylates lysine 9 of histone H3 (H3K9me3), a hallmark of transcriptional repression. Related references: PMID:11959841, PMID:12869583, PMID:14536086
• It functions primarily in euchromatin to silence genes, coupling H3K9me3 with DNA methylation through interactions with MBD1 and ATF7IP, and this repression is heritably maintained during DNA replication via CAF-1. Related references: PMID:12869583, PMID:14536086, PMID:27237050
• SETDB1 is recruited to chromatin by TRIM28/KAP1, which is directed by KRAB zinc-finger proteins, and is essential for HUSH-mediated heterochromatin formation and gene silencing. Related references: PMID:24623306, PMID:27732843
• In embryonic stem cells, SETDB1 maintains repression of genes and endogenous retroviruses, cooperating with TRIM28 for H3K9me3 deposition independently of DNA methylation. Related references: PMID:24623306, PMID:37938770
• SETDB1 also catalyzes mono- and dimethylation of H3K9 in the cytoplasm on free histones prior to chromatin assembly. Related references: PMID:26405197
• It localizes predominantly to the nucleus but is also found in the cytoplasm and associates with chromosomes.
• An alternatively spliced isoform lacks key methyltransferase domains and is catalytically inactive.
• Widely expressed with highest levels in testis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–397); the antibody may recognize the full-length SETDB1 protein (~143 kDa) and possibly N-terminal fragments.
• KO validation suggests high specificity; researchers should include knockout or knockdown controls in their experimental setup to confirm specificity in their own systems.
• For Western blotting, use a high-molecular-weight marker range and consider long transfer times or low-percentage gels for the 143 kDa protein.
• For immunofluorescence/IF-P, optimize fixation and permeabilization protocols to detect nuclear/chromosomal SETDB1.
• In ELISA, verify cross-reactivity if using species other than human, mouse, or rat.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete range of IVD raw materials for epigenetic research, including validated anti-SETDB1 antibodies for applications like WB, IF, and ELISA. We support diagnostic manufacturers and researchers with bulk antibody supplies, optimized antibody pairs, ancillary reagents, and customized sourcing for rare targets, helping you accelerate from concept to clinic with reliable quality and service.