Anti-SETD6 Polyclonal Antibody for WB, ELISA - Q8TBK2

Core Product Specifications and Parameters

Biological Background: SETD6 Function and Localization

• SETD6, also known as SET domain-containing protein 6, is an N-lysine methyltransferase that catalyzes the mono-methylation of specific lysine residues on target proteins, modulating their functions.
• It monomethylates Lys-310 of the RELA subunit of the NF-kappa-B complex, leading to down-regulation of NF-kappa-B transcription factor activity. Related references: PMID:21131967 PMID:21515635 PMID:30189201
• SETD6 also monomethylates Lys-8 of histone variant H2AZ (H2AZK8me1), contributing to chromatin regulation. Related references: PMID:23324626
• It methylates PAK4, although the functional significance of this modification is still being characterized.
• The protein is required for the maintenance of embryonic stem cell self-renewal, as indicated by similarity studies.
• Localized predominantly in the nucleus, SETD6 operates in the nucleoplasm to exert its methyltransferase activity.
• Classified as a transferase using S-adenosyl-L-methionine as a methyl donor, it is subject to alternative splicing and has a molecular weight of approximately 53 kDa (UniProt: 53189 Da).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 301–473 of human SETD6; therefore, the antibody may recognize epitopes within this region.
• For Western blot (WB), the predicted molecular weight is approximately 53 kDa; validate the band size in your sample and consider appropriate controls.
• This antibody has been reported to react with human, mouse, and rat samples; cross-reactivity with other species should be individually assessed.
• For ELISA applications, titrate the antibody concentration to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams by providing validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing solutions. Our anti-SETD6 polyclonal antibody, targeting a key epigenetic regulator, is part of our extensive portfolio of reliable immunoassay reagents. Contact us to discuss your specific IVD raw-material requirements, from discovery to clinic.