Anti-SETD5 Rabbit Polyclonal Antibody for WB - Q9C0A6

Core Product Specifications and Parameters

Biological Background: SETD5 Function and Localization

• SETD5 (SET domain-containing protein 5) is a histone-lysine N-methyltransferase, also referred to as MRD23 or SETD5A, encoded by the SETD5 gene.
• It functions as a chromatin regulator critical for brain development, modulating RNA elongation rate to control neural stem cell proliferation and synaptic transmission.
• SETD5 may catalyze trimethylation of histone H3 at lysine 36 (H3K36me3), a modification essential for proper RNA elongation dynamics.
• In vitro, it also exhibits H3K9 monomethylation (H3K9me1) activity, though the biological relevance of its methyltransferase activity remains under discussion.
• The protein localizes to the nucleus and associates with chromosomes, consistent with its role in transcription regulation.
• SETD5 is classified as a transferase and is listed as a phosphoprotein; it is implicated in neurodevelopmental disorders, including autism spectrum disorder and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1054-1153 of human SETD5 (NP_001073986.1), a region near the C-terminus, which may influence isoform specificity.
• For western blot (WB), expect a band near 158 kDa; consider using appropriate loading controls and validate in the relevant cell or tissue lysates.
• In immunohistochemistry (IHC-P), brain tissue sections are recommended due to high SETD5 expression; include proper fixation and antigen retrieval steps.
• Immunofluorescence (IF/ICC) may require optimization of permeabilization and blocking conditions to reduce background.
• ELISA protocols should incorporate a standard curve and appropriate dilutions; cross-reactivity with mouse and rat orthologs suggests the antibody can be used across these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise in chromatin regulation and neurodevelopmental targets enables efficient sourcing of rare and specialized raw materials. Contact CamelBio to discuss your specific SETD5 antibody requirements and broader project needs.