Anti-SETD5 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q9C0A6

Core Product Specifications and Parameters

Biological Background: SETD5 Function and Localization

• SETD5 (SET domain-containing protein 5) is a histone-lysine N-methyltransferase that catalyzes methylation of histone H3 at Lys-36 (H3K36me3) and, in vitro, at Lys-9 (H3K9me1). Its methyltransferase activity is subject to discussion.
• It functions as a chromatin regulator essential for brain development, controlling neural stem cell proliferation and synaptic transmission by regulating RNA elongation rate.
• The protein localizes to the nucleus and associates with chromosomes.
• SETD5 is implicated in neurodevelopmental disorders; mutations are associated with intellectual disability and autism spectrum disorder.
• Alternative splicing yields multiple isoforms, and the protein undergoes phosphorylation.
• Its molecular weight is approximately 158 kDa (UniProt: 157.5 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 520–740 of human SETD5, a region likely conserved across major isoforms. Validate reactivity in relevant human cell lines or tissues.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization to detect nuclear localization. Consider using a nuclear counterstain for colocalization.
• For ELISA, test the antibody as capture or detection reagent after confirming specificity with recombinant SETD5 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and technical support. This anti-SETD5 polyclonal antibody targets a chromatin regulator critical for neurodevelopmental research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic.