Anti-SETD3 Polyclonal Antibody for WB, IP, ELISA - Q86TU7

Core Product Specifications and Parameters

Biological Background: SETD3 Function and Localization

• SETD3, also known as hSETD3 or C14orf154, is a SET domain-containing protein 3 encoded by the SETD3 gene.
• SETD3 is an actin-histidine N-methyltransferase that specifically methylates actin at histidine-73 (His-73) to form 3-methylhistidine (tele-methylhistidine). This activity is critical for proper actin dynamics. Related references: PMID:30526847, PMID:30626964, PMID:30785395
• Histidine methylation of actin by SETD3 is required for smooth muscle contraction, particularly in the laboring uterus during delivery, highlighting its role in physiological processes. Related references: PMID:30626964
• The enzyme exhibits strict substrate specificity; it does not possess protein-lysine N-methyltransferase activity and likely only methylates actin at His-73, underscoring its unique functional niche. Related references: PMID:30626964, PMID:30785395, PMID:31388018
• SETD3 localizes to both the cytoplasm and nucleus, suggesting possible compartment-specific functions beyond actin methylation.
• The protein is subject to alternative splicing, phosphorylation, and ubiquitin conjugation, and contains a SET domain that binds S-adenosyl-L-methionine, the methyl donor for its transferase activity.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide spanning amino acids 72–329 of human SETD3. This region covers the N-terminal portion and part of the SET domain; thus, the antibody is expected to recognize full-length SETD3 (predicted molecular weight ~67 kDa).
• For Western blotting (WB), it is advisable to run appropriate positive and negative control lysates (e.g., SETD3-expressing vs. knockdown cell lines) to confirm specificity. Detection of a band at ~67 kDa in human samples is expected.
• In immunoprecipitation (IP) assays, antibody concentration and incubation time may need optimization; a standard starting point is 1–2 µg per 500 µg of lysate.
• When performing ELISA, consider using recombinant SETD3 protein or peptide fragments as coating antigens and titrate the antibody for optimal signal-to-noise ratio.
• Since cross-reactivity is indicated for human, validation in other species should be carried out independently if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a leading IVD raw-material partner, offering comprehensive sourcing solutions from early-stage R&D to clinical application. With this anti-SETD3 polyclonal antibody, researchers can accelerate investigations into actin methylation and smooth muscle biology. Beyond single reagents, we provide validated antibody pairs, bulk monoclonals/polyclonals, and customized sourcing for rare targets—ensuring reliable supply chains for diagnostic and life science projects.