Anti-SETD2 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q9BYW2

Core Product Specifications and Parameters

Biological Background: SETD2 Function and Localization

• SETD2 is a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3), representing the main enzyme generating this mark for epigenetic transcriptional activation. It can utilize dimethylated H3K36 as substrate and also methylate unmethylated H3K36 in vitro. Related references: PMID:16118227, PMID:19141475, PMID:21526191, PMID:19332550
• Plays a critical role in chromatin structure modulation during transcription elongation by coordinating recruitment of the FACT complex and interacting with hyperphosphorylated RNA polymerase II (POLR2A). Related references: PMID:23325844
• Acts as a key regulator of DNA mismatch repair in G1 and early S phase: H3K36me3 generated by SETD2 is required to recruit the MSH6 subunit of the MutS alpha complex to chromatin, enabling rapid mismatch identification. Related references: PMID:23622243
• Required for DNA double-strand break repair by homologous recombination; mediates H3K36me3 formation at break sites to promote RAD51 recruitment. Related references: PMID:24843002
• Functions as a tumor suppressor, and H3K36me3 also recruits DNA methyltransferase DNMT3A to maintain heterochromatic states. Related references: PMID:24509477, PMID:27317772
• SETD2 recruitment and H3K36me3 are enhanced in intron-containing genes, suggesting coupling of splicing to transcription elongation. Related references: PMID:21792193
• In addition to histones, mediates methylation of alpha-tubulin (Lys-40) and STAT1 (Lys-525), contributing to normal mitosis, cytokinesis, and interferon-alpha-induced antiviral defense. Related references: PMID:27518565, PMID:28753426
• Subcellular localization is primarily nuclear, associated with chromosomes. Ubiquitously expressed across tissues.

Experimental Guidance and Technical Tips

• For western blot (WB) detection, consider using a low-percentage gel (e.g., 6% or gradient) to effectively resolve the high molecular weight SETD2 protein (~288 kDa).
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), standard antigen retrieval and permeabilization methods may be necessary for optimal signal; validate in your relevant sample system.
• The immunogen peptide maps to a C‑terminal region (aa 2475‑2564) of human SETD2; while cross‑reactive with mouse and rat, confirm reactivity in your species of interest due to possible sequence variations.
• For immunoprecipitation (IP), pre‑clear lysates and include appropriate isotype controls to minimize nonspecific binding.
• Always include positive and negative controls (e.g., lysates with known SETD2 expression levels) to validate detection in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-SETD2 polyclonal antibody addresses critical needs in epigenetics, DNA repair, and cancer research, and CamelBio can further support your development pipeline by sourcing rare‑target antibodies and related raw materials to help accelerate your assay development. Our one‑stop procurement model ensures you receive consistent quality and scale, backed by technical consultation throughout your product lifecycle.