Anti-SETD2 Polyclonal Antibody for WB, ELISA - Q9BYW2

Core Product Specifications and Parameters

Biological Background: SETD2 Function and Localization

• SETD2 is a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3), a mark associated with transcriptional activation. Related references: PMID:16118227, PMID:19141475, PMID:21526191
• It is the primary H3K36me3 methyltransferase, coordinating FACT complex recruitment and interacting with hyperphosphorylated RNA polymerase II to modulate chromatin during elongation. Related references: PMID:23325844
• SETD2-mediated H3K36me3 is critical for DNA mismatch repair in G1/early S phase by recruiting the MutSα complex to chromatin. Related references: PMID:23622243
• Required for DNA double-strand break repair via homologous recombination, promoting RAD51 recruitment at damage sites. Related references: PMID:24843002
• Acts as a tumor suppressor and is frequently mutated in various cancers. Related references: PMID:24509477
• Also methylates non-histone substrates, including α-tubulin (Lys-40) and STAT1 (Lys-525), influencing mitosis, cytokinesis, and antiviral interferon responses. Related references: PMID:27518565, PMID:28753426
• Ubiquitously expressed and localizes to the nucleus and chromosomes.
• Contains a SET domain and multiple functional motifs; functions as a chromatin regulator and transcriptional activator.

Experimental Guidance and Technical Tips

• For Western blot, use a high-molecular-weight marker and extended transfer times to ensure efficient detection of the 288 kDa SETD2 protein.
• Include appropriate positive and negative controls (e.g., HeLa cell lysates, SETD2 knockdown samples) to confirm band specificity.
• When performing ELISA, consider titrating the antibody against the immunogen or full-length protein to determine optimal coating and detection conditions.
• Given the polyclonal nature, each new lot should be validated in the relevant human sample system to confirm consistent reactivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials and technical services—from early development to clinical application. This SETD2 antibody is well-suited for epigenetic profiling and oncology research, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare-target sourcing. Contact us to streamline your procurement and accelerate your project timeline.