Anti-SETD1B Rabbit Polyclonal Antibody for WB and ELISA - Q9UPS6

Core Product Specifications and Parameters

Biological Background: SETD1B Function and Localization

• SETD1B (also designated KMT2G, Set1B, or IDDSELD) is a histone-lysine N-methyltransferase that catalyzes the transfer of methyl groups from S-adenosyl-L-methionine to the ε-amino group of histone H3 lysine 4 (H3K4), generating mono-, di-, and trimethylated H3K4 (H3K4me1/2/3) marks via a non-processive mechanism (PubMed:17355966, PubMed:25561738).
  Related references: PMID:17355966 PMID:25561738
• The H3K4me1/2/3 modifications are deposited at transcriptionally active chromatin sites, where they facilitate gene expression and DNA repair (PubMed:17355966, PubMed:25561738).
  Related references: PMID:17355966 PMID:25561738
• SETD1B plays an essential role in hematopoiesis, regulating the transcriptional programming of multipotent hematopoietic progenitors and lymphoid lineage specification (By similarity).
• It localizes to the nucleus, nuclear speckles, chromosomes, and cytoplasm, consistent with functions in chromatin modification and potential extranuclear activities.
• Disease-associated variants of SETD1B are linked to epilepsy and intellectual disability, as highlighted by UniProt keywords.
• The protein is subject to phosphorylation and alternative splicing, contributing to its functional regulation.
• SETD1B possesses RNA-binding capability and can act as a transcriptional activator, indicating multifunctional roles in gene regulation.

Experimental Guidance and Technical Tips

• Western blotting: Expect a band near the predicted molecular weight of 213 kDa. Use low-percentage SDS-PAGE gels and extended transfer times to ensure efficient transfer of large proteins. Validate detection in the appropriate sample type (e.g., whole cell lysates).
• ELISA: The antibody may be suitable as a capture or detection reagent; optimize coating concentrations and assay conditions empirically.
• Cross-reactivity: This antibody recognizes human SETD1B and shows reactivity with mouse and rat orthologs. Verify performance in your species of interest if using for quantitative assays.
• Immunogen design: The antibody was raised against a recombinant protein fragment spanning amino acids 1673–1770 of human SETD1B (NP_001340274.1), which may correspond to a region outside structurally conserved domains. Consider this when mapping epitopes or designing peptide competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a reliable supply of this anti-SETD1B rabbit polyclonal antibody, ideal for investigations into chromatin regulation and hematopoiesis. Our one-stop sourcing platform provides diagnostic manufacturers and laboratories with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With comprehensive technical services and consulting, we support your journey from early research to clinical applications.