Anti-SETBP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6X0

Core Product Specifications and Parameters

Biological Background: SETBP1 Function and Localization

• SETBP1 (SET-binding protein 1) is a nuclear protein encoded by the human gene SETBP1 (Gene ID: 26040).
• Contains a repetitive sequence motif characteristic of the SET-binding family.
• Functions as a DNA-binding protein that interacts with the SET nuclear oncogene, potentially involved in chromatin regulation and gene expression.
• Expressed ubiquitously in human tissues, with relatively low levels in normal myeloid and monocytic cells and CD34+ progenitors, but elevated expression is observed in myeloid malignancies.
• Associated with disease variants, including autosomal dominant intellectual disability (MRD29).
• Undergoes alternative splicing and post-translational acetylation.
• Predicted molecular weight of approximately 175 kDa, consistent with detection in western blot.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1–242) of human SETBP1; this epitope may allow detection of full-length and certain splice variants.
• For western blot, expect a band near 175 kDa; use a low-percentage SDS-PAGE gel (e.g., 6–8%) and optimize transfer conditions for high-molecular-weight proteins.
• Validate performance in relevant human sample systems; leukemia cell lines or primary cells with known SETBP1 overexpression are suggested as positive controls.
• In immunofluorescence/ICC, anticipate nuclear staining; consider co-staining with a nuclear marker for confirmation.
• ELISA applications may employ direct or indirect formats; titrate the antibody to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, supporting diagnostic manufacturers and research labs with high-quality reagents and comprehensive technical services. This anti-SETBP1 polyclonal antibody is part of our extensive portfolio for oncology and genetic disorder research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibody resources, bulk ancillary reagents, and custom sourcing for rare targets, helping you accelerate every stage from concept to clinic.