Anti-SET1A Polyclonal Antibody for WB, ELISA - O15047

Core Product Specifications and Parameters

Biological Background: SET1A Function and Localization

• SET1A, also known as KMT2F or Set1/Ash2 histone methyltransferase complex subunit SET1, is a histone-lysine N-methyltransferase that specifically mono-, di-, and trimethylates 'Lys-4' of histone H3 (H3K4me1, me2, me3) using S-adenosyl-L-methionine as a methyl donor (PubMed:12670868, PubMed:25561738). Related references: PMID:12670868 PMID:25561738
• It is a core component of the chromatin remodeling machinery, establishing H3K4 methylation marks at active chromatin sites to regulate transcription and DNA repair processes (PubMed:29937342, PubMed:31197650, PubMed:32346159). Related references: PMID:29937342 PMID:31197650 PMID:32346159
• SET1A is essential for H3K4me3 enrichment at promoters and transcriptional programming of inner cell mass stem cells and neuron progenitors during embryogenesis (By similarity) (PubMed:31197650). Related references: PMID:31197650
• At stalled replication forks, SET1A catalyzes H3K4me1 and mediates FANCD2-dependent nucleosome remodeling, stabilizing RAD51 nucleofilaments to protect reversed forks from degradation (PubMed:29937342, PubMed:32346159). Related references: PMID:29937342 PMID:32346159
• SET1A does not methylate H3K4 if the neighboring 'Lys-9' residue is already methylated, indicating substrate specificity (PubMed:12670868). Related references: PMID:12670868
• It binds various RNAs involved in RNA processing and the DNA damage response, suggesting a role in linking chromatin modification to RNA metabolism (PubMed:38003223). Related references: PMID:38003223
• Subcellularly, SET1A localizes to the nucleus speckles, chromosomes, and also the cytoplasm, with its key functions executed at chromatin sites.
• It belongs to the class V-like SAM-binding methyltransferase superfamily and is implicated in neurodevelopmental disorders, as mutations are associated with epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• For Western blot, a band around 186 kDa is expected; consider using a high-molecular-weight transfer protocol and appropriate positive controls (e.g., nuclear extracts from human or mouse cell lines).
• For ELISA, the antibody may be used to detect recombinant or native SET1A; optimization of coating antigen and detection conditions is recommended.
• Due to high molecular weight, extended transfer time and low-percentage gels may improve detection in WB.
• Cross-reactivity with mouse suggests utility in murine model studies; validate reactivity in the desired tissue/cell type.
• As a polyclonal antibody, lot-to-lot consistency should be assessed for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material partner, connecting diagnostic manufacturers, laboratories, and research institutes with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For epigenetic and chromatin research areas, including histone modification targets like SET1A, we offer comprehensive supply solutions to accelerate your assay development from concept to clinic.