Anti-SESN3 Polyclonal Antibody for WB, ELISA - P58005

Core Product Specifications and Parameters

Biological Background: SESN3 Function and Localization

• Sestrin-3 (SESN3), also known as SEST3, is a member of the sestrin family of stress-responsive proteins.
• It functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway, linking amino acid availability to cell growth control. Related references: PMID:25263562
• May regulate the insulin-receptor signaling pathway through activation of TORC2, influencing metabolic homeostasis.
• Plays a role in protection against oxidative and genotoxic stresses, likely via its N-terminal alkylhydroperoxide reductase activity that prevents accumulation of reactive oxygen species (ROS).
• Localizes to the cytoplasm, consistent with its role in nutrient sensing and stress responses.
• Widely expressed across human tissues, reflecting its fundamental role in cellular metabolism.
• Features alternative splicing (as indicated by UniProt keywords) and is classified as an oxidoreductase.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–310) covers a large N-terminal region of human SESN3; ensure this region is conserved in the target sample to confirm antibody reactivity.
• For western blot, validate the antibody using whole cell lysates from human cell lines; SESN3 is widely expressed, but a positive control expressing the target at ~57 kDa is recommended.
• Include appropriate negative controls (e.g., knockout or siRNA-treated samples) to confirm band specificity.
• For ELISA, consider coating the plate with recombinant SESN3 protein or cell lysate; optimize coating concentrations and antibody dilutions empirically.
• Due to alternative splicing, verify that the detected band corresponds to the expected molecular weight; potential isoforms could differ in size.
• Shipping on ice bags suggests stability at cold temperatures; aliquot antibody upon receipt and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a comprehensive portfolio of IVD raw materials. For metabolic and oxidative stress research targeting SESN3, we can supply validated antibody pairs, bulk monoclonals/polyclonals, and ancillary reagents tailored to your assay development needs. With a focus on bridging from concept to clinic, CamelBio is your trusted partner for rare-target raw-material sourcing and supply chain continuity.