Anti-Ser/thr-PP2A activator (PTPA) Polyclonal Antibody for WB, IF/ICC, ELISA - Q15257

Core Product Specifications and Parameters

Biological Background: Ser/thr-PP2A activator (PTPA) Function and Localization

• PTPA acts as a regulatory subunit of serine/threonine-protein phosphatase 2A (PP2A), modulating its activity or substrate specificity by inducing conformational changes in the catalytic subunit. Related references: PMID:16916641 PMID:36073231
• As a peptidyl-prolyl cis-trans isomerase (PPIase), it accelerates protein folding by catalyzing proline imidic bond isomerization in oligopeptides (By similarity).
• PTPA can reactivate inactive PP2A holoenzymes (PP2A(i)) in vitro in the presence of ATP and Mg²⁺ (By similarity).
• It reversibly stimulates the phosphotyrosyl phosphatase activity of PP2A core heterodimers (PP2A(D)), an activity that depends on the associated ATPase activity of the complex. Related references: PMID:16916641
• Beyond its phosphatase regulatory roles, PTPA is involved in apoptosis, functioning independently of PP2A in this pathway. Related references: PMID:17333320
• The protein localizes to both the cytoplasm and nucleus.
• It is widely expressed across human tissues and is subject to acetylation; alternative splicing produces multiple isoforms. Disease associations include intellectual disability, Parkinson disease, and neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full N-terminal domain (residues 1–323) of human PTPA (NP_821067.1); consider this region when designing orthogonal validation experiments.
• For Western blot, expect a band around 41 kDa in lysates from human, mouse, or rat tissues/cells; validate specificity with appropriate controls.
• In immunofluorescence/ICC, account for the cytoplasmic and nuclear distribution of PTPA; co-staining with compartment-specific markers may aid interpretation.
• ELISA applications can be used for qualitative or relative quantification of PTPA in biological fluids or cell extracts; optimize coating and detection conditions experimentally.
• Because PTPA regulates PP2A and apoptosis, functional studies may benefit from including ATP/Mg²⁺ or phosphatase activity assays in the experimental design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with trusted manufacturers to supply high-quality rabbit polyclonal antibodies targeting PTPA, suitable for Western blot, IF/ICC, and ELISA. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials to accelerate your diagnostic assay development. Our integrated sourcing services cover serine/threonine phosphatase research needs, supporting everything from concept to clinical application.