Anti-SERPINI2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - O75830

Core Product Specifications and Parameters

Biological Background: Serpin I2 Function and Localization

• SERPINI2 (Serpin I2) is also designated as Myoepithelium-derived serine protease inhibitor (MEPI), Pancpin, Pancreas-specific protein TSA2004, and Peptidase inhibitor 14 (PI-14).
• It belongs to the serpin (serine protease inhibitor) superfamily and acts as a serine protease inhibitor.
• The protein is predicted to be secreted, containing a signal peptide for extracellular release.
• SERPINI2 is classified as a glycoprotein, as indicated by the Glycoprotein keyword.
• Tissue expression is primarily observed in the pancreas and adipose tissues.
• Its molecular weight is approximately 46 kDa (UniProt: 46145 Da).
• SERPINI2 is part of the UniProt reference proteome and has been identified in proteomic analyses.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a recombinant immunogen spanning amino acids 56-405 of human SERPINI2, covering the core serpin domain; detectability of endogenous protein should be verified in relevant lysates.
• Validated applications include Western blot (WB), immunofluorescence on paraffin-embedded sections (IF-P), and ELISA. Optimize antibody dilution and conditions for each assay type.
• Cross-reactivity has been observed with mouse and rat orthologs; consider sequence homology and run appropriate controls when employing non-human samples.
• For WB, expect a band near 46 kDa; run a positive control (e.g., pancreas or adipose tissue lysate) to confirm specificity.
• For IF-P, due to the secreted nature of SERPINI2, evaluate permeabilization and fixation protocols to capture both intracellular and extracellular protein pools.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, technical services, and consulting from concept to clinic. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your diagnostic and research pipelines. This anti-SERPINI2 antibody addresses protease inhibitor and pancreatic biology applications, and our team can facilitate scaling from proof-of-concept to commercial supply.