Anti-SERPING1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05155

Core Product Specifications and Parameters

Biological Background: SERPING1 Function and Localization

• SERPING1, also referred to as C1 inhibitor (C1INH), C1 esterase inhibitor, or serpin G1, is a secreted serine protease inhibitor of the serpin superfamily, playing a critical role in controlling proteolytic cascades in plasma.
• It is the primary regulator of the classical complement pathway, forming irreversible, proteolytically inactive stoichiometric complexes with the activated C1r and C1s proteases.
  Related references: PMID:10946292 PMID:3458172 PMID:6416294
• Beyond complement, SERPING1 regulates blood coagulation and the contact system by potently inhibiting factor XIIa (FXIIa), and it also inhibits chymotrypsin and plasma kallikrein, thereby modulating fibrinolysis and kinin generation.
  Related references: PMID:8495195
• The protein is secreted into the bloodstream and other body fluids, consistent with its role as a systemic protease inhibitor.
• SERPING1 undergoes alternative splicing and extensive post‑translational modifications, including glycosylation and disulfide bond formation; mutations in the SERPING1 gene are linked to hereditary angioedema types I and II.
• A functional C1 inhibitor is essential for maintaining hemostatic and inflammatory balance, with its deficiency or dysfunction leading to uncontrolled complement and contact pathway activation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full mature protein (residues 23–500), lacking only the signal peptide; thus, the polyclonal antibody is expected to recognize multiple epitopes across the molecule, suitable for detecting both full‑length and potentially processed forms.
• For Western blotting, a band around 55 kDa is expected under denaturing and reducing conditions; however, due to glycosylation and alternative splicing, validate the observed molecular weight in your sample system.
• For immunofluorescence/immunocytochemistry, consider appropriate fixation and permeabilization protocols, given the secreted nature of SERPING1; co‑staining with an endoplasmic reticulum/Golgi marker may aid localization studies.
• The antibody shows cross‑reactivity with mouse and rat, enabling translational research; nonetheless, always confirm reactivity in the specific species and tissue of interest before quantitative assays.
• ELISA performance may require optimization of coating and detection conditions; consider pairing with a validated capture antibody if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supplying high‑quality antibodies, antigens, and ancillary reagents to diagnostic manufacturers and research laboratories worldwide. For complement and coagulation research, we offer validated polyclonal and monoclonal antibodies targeting key regulators such as SERPING1, along with recombinant proteins and bulk reagent services. Our one‑stop platform supports your workflow from early concept through clinical application, providing technical and logistics support for rare‑target raw‑material procurement.