Anti-SERPING1 Rabbit Monoclonal Antibody for WB and ELISA - P05155

Core Product Specifications and Parameters

Biological Background: SERPING1 Function and Localization

• The SERPING1 gene encodes plasma protease C1 inhibitor (C1-INH), also known as C1 esterase inhibitor or Serpin G1, a serine protease inhibitor (serpin) that is a key regulator of the complement system.
• C1-INH irreversibly inhibits C1r and C1s proteases of the classical complement pathway by forming stable, proteolytically inactive stoichiometric complexes. Related references: PMID:10946292 PMID:3458172 PMID:6416294
• Beyond complement, SERPING1 modulates blood coagulation, fibrinolysis, and kinin generation; it is a highly effective inhibitor of coagulation factor XIIa (FXIIa) and also inhibits chymotrypsin and kallikrein. Related references: PMID:8495195
• The protein is secreted into the blood and is essential for controlling unwanted activation of inflammatory and hemostatic pathways.
• Key post-translational modifications include glycosylation, and the protein contains disulfide bonds characteristic of the serpin family.
• Alternative splicing of SERPING1 yields multiple isoforms, and mutations in the gene are associated with hereditary angioedema (HAE) types 1 and 2.
• Subcellular localization is primarily secreted, consistent with its role as a circulating plasma inhibitor.
• The molecular weight of the mature protein is approximately 55 kDa, aligning with the observed protein weight in standard assays.

Experimental Guidance and Technical Tips

• For Western blotting, the rabbit monoclonal format ensures high specificity; consider validating the signal in relevant cell lines or tissues known to express SERPING1, such as liver-derived cells, using the predicted band around 55 kDa.
• For ELISA, this antibody can be used as a capture or detection reagent; optimize coating concentrations and consider pairing with a matched detection antibody for sandwich ELISA.
• The immunogen design targeting the central region (aa 150-250) may recognize multiple isoforms or post-translationally modified forms; verify reactivity in your specific application.
• Cross-reactivity with mouse and rat SERPING1 is reported; validate in the relevant sample system to ensure comparable performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing by offering validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and tailored procurement for rare targets. This SERPING1 antibody supports complement research and diagnostic development, and CamelBio can assist with integrated supply solutions for your assay workflows.