Anti-SERPINF2 Rabbit Polyclonal Antibody for WB and ELISA - P08697

Core Product Specifications and Parameters

Biological Background: Alpha-2-antiplasmin Function and Localization

• Protein identity and function: Alpha-2-antiplasmin (SERPINF2) is a serine protease inhibitor (serpin) that primarily targets plasmin and trypsin, and also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
• Role in fibrinolysis: It is the main physiological inhibitor of plasmin, thereby regulating clot lysis and maintaining hemostasis.
• Gene and isoforms: Encoded by the SERPINF2 gene, alternative splicing yields multiple isoforms; this antibody targets a region common to isoform 1 (precursor).
• Subcellular localization: Secreted protein found in blood plasma.
• Tissue expression: Expressed predominantly by the liver and secreted into the circulation.
• Post-translational modifications and features: Glycoprotein with disulfide bonds and sulfation; belongs to the serpin superfamily.
• Clinical relevance: Acts as an acute phase protein and is linked to bleeding disorders when deficient.

Experimental Guidance and Technical Tips

• Immunogen design: The immunogen corresponds to amino acids 302-491, a region within the C-terminal portion of SERPINF2. This design may recognize both full-length protein and certain truncated isoforms.
• Western blot (WB): A band around ~55 kDa is expected in reducing conditions. We recommend using plasma or liver lysates as positive controls and optimizing antibody dilution.
• ELISA: Suitable for detecting alpha-2-antiplasmin in capture or indirect ELISA formats; pairing with a detection antibody may require validation in your assay system.
• Cross-reactivity: Confirmed to recognize human, mouse, and rat SERPINF2; consider sequence alignment for other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement with a comprehensive portfolio that includes this anti-SERPINF2 rabbit polyclonal antibody for coagulation and fibrinolysis research. From validated monoclonal/polyclonal antibodies and matched antibody pairs to bulk ancillary reagents and rare target sourcing, our one-stop platform supports diagnostic manufacturers and labs from concept to clinical application. Contact us to explore customized solutions for your specific assay development needs.