Anti-SERPINE2 Rabbit Polyclonal Antibody for WB, ELISA - P07093

Core Product Specifications and Parameters

Biological Background: SERPINE2 Function and Localization

• SERPINE2 encodes Glia-derived nexin, also known as Protease nexin 1 (PN-1), Protease nexin I, Peptidase inhibitor 7 (PI-7), and Serpin E2.
• It functions as a serine protease inhibitor with activity toward thrombin, trypsin, and urokinase, playing a key role in regulating proteolytic cascades.
• By inhibiting thrombin, SERPINE2 promotes neurite extension, implicating it in neurogenesis and neuronal differentiation.
• The protein binds heparin, which may modulate its activity and localization within the extracellular matrix.
• Subcellular localization is predominantly secreted and extracellular, consistent with its role in extracellular proteolysis control.
• Additional features include glycoprotein modification, alternative splicing, and direct protein sequencing evidence, highlighting its post-translational complexity.
• Keywords associated with SERPINE2 include developmental protein, differentiation, protease inhibitor, and secreted signal, reflecting its functional diversity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 20–398 of human SERPINE2 (NP_006207.1), representing the majority of the mature protein after signal peptide cleavage. This design likely yields broad epitope recognition.
• For Western blot, expect a band near 44 kDa under reducing conditions. Validate in the relevant cell or tissue lysate system, considering potential glycosylation that may alter migration.
• This antibody is suitable for ELISA; optimization of coating antigen and antibody concentrations may be required.
• Cross-reactivity with mouse SERPINE2 has been confirmed, enabling translational studies in mouse models. For other species, sequence homology analysis and experimental validation are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this anti-SERPINE2 polyclonal antibody, to support diagnostic development and research in protease biology and neuroscience. Our one-stop sourcing model provides access to optimized antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target procurement services, helping manufacturers and labs accelerate their assay development from concept to clinic.