Anti-SERPINE2 Polyclonal Antibody for WB, ELISA - P07093

Core Product Specifications and Parameters

Biological Background: SERPINE2 Function and Localization

• SERPINE2, also known as Glia-derived nexin or Protease nexin-1 (PN-1), is a member of the serpin superfamily, which functions as serine protease inhibitors.
• It potently inhibits serine proteases such as thrombin, trypsin, and urokinase, thereby regulating extracellular proteolytic activity.
• In the nervous system, SERPINE2 promotes neurite outgrowth and neuronal differentiation by blocking thrombin-mediated neurite retraction.
• The protein is secreted into the extracellular space and binds heparin, a property that may influence its localization and activity in the extracellular matrix.
• Alternative splicing generates multiple isoforms, potentially contributing to tissue-specific functions.
• Keywords from UniProt highlight roles in development, neurogenesis, differentiation, and heparin binding, consistent with its pleiotropic functions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 27–398, covering nearly the full-length mature protein, which may yield broad epitope recognition; validate specificity for your target isoform and species.
• In Western blot, the predicted molecular weight is ~44 kDa, but glycosylation may cause a higher apparent mass; optimize gel acrylamide percentage and run appropriate controls.
• The antibody cross-reacts with mouse and rat according to the supplier; confirm performance in relevant tissue or cell lysates from these species.
• For ELISA, use the antibody as a capture or detection reagent after empirical determination of optimal coating and antibody concentrations.
• As SERPINE2 is a secreted protein, consider analyzing conditioned media or extracellular preparations to enhance detection in functional assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete range of IVD raw materials for serine protease and neurobiology research, including this high-quality SERPINE2 polyclonal antibody. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and efficient sourcing for rare targets. Partner with us to streamline your diagnostic development from concept to clinic.