Anti-SERPINC1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P01008

Core Product Specifications and Parameters

Biological Background: Antithrombin-III Function and Localization

• Antithrombin-III (AT-III), encoded by the SERPINC1 gene, is a member of the serpin superfamily and the most important serine protease inhibitor in plasma, playing a critical role in regulating the blood coagulation cascade. Related references: PMID:15140129, PMID:15853774
• AT-III inhibits several procoagulant proteases, including thrombin, matriptase-3/TMPRSS7, and factors IXa, Xa, and XIa, thereby maintaining hemostatic balance.
• The inhibitory activity of AT-III is markedly enhanced in the presence of heparin, a property that underpins its therapeutic use in thrombotic disorders.
• Antithrombin-III is a secreted glycoprotein found primarily in blood plasma, consistent with its role in extracellular coagulation regulation.
• Post-translational modifications of AT-III include disulfide bond formation, glycosylation, and phosphorylation, which may influence its stability and function.
• Mutations in the SERPINC1 gene are associated with thrombophilia, highlighting its essential role in preventing excessive blood clotting.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human SERPINC1, a region at the N-terminus; this design may recognize both native and denatured forms of the protein, though validation in the relevant sample system is advised.
• For Western blot, the expected molecular weight for human SERPINC1 is approximately 58kDa (as per product specifications); however, actual migration may differ due to glycosylation or sample conditions, so molecular weight markers should be used for confirmation.
• In IHC-P applications, antithrombin-III is primarily localized to plasma and extracellular spaces; consider using appropriate positive controls (e.g., liver or plasma-rich tissues) and validate staining patterns against known expression.
• For ELISA, this antibody may be paired with a suitable detection antibody; conducting cross-reactivity and matrix effect studies using the intended sample matrix (e.g., plasma, serum) is recommended.
• Given the cross-reactivity with mouse and rat, this antibody may be useful for preclinical studies, but experiments should be performed to confirm reactivity in the specific models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories a comprehensive sourcing solution for IVD raw materials, with a focus on coagulation and hemostasis targets such as antithrombin-III (SERPINC1). Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents delivered in bulk, enabling streamlined procurement from discovery to clinical application.