Anti-SERPINB13 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UIV8

Core Product Specifications and Parameters

Biological Background: SERPINB13 Function and Localization

• SERPINB13, also known as Hurpin, Headpin, or Peptidase inhibitor 13, is a member of the serpin (serine protease inhibitor) superfamily.
• It is encoded by the SERPINB13 gene (GeneID: 5275) in humans, and the protein is also referred to as PI-13 or HaCaT UV-repressible serpin.
• The protein may play a role in the proliferation or differentiation of keratinocytes, the predominant cell type in the epidermis.
• It is predominantly localized to the cytoplasm, consistent with its function as an intracellular protease inhibitor.
• Expression is skin-specific, with high levels in keratinocytes, and it is downregulated by UV irradiation in HaCaT cells.
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversity.
• As a serpin, it likely inhibits serine proteases, although its specific target proteases remain to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the N-terminal region (amino acids 1-140). Verify antibody specificity using peptide blocking or knockout/knockdown controls.
• For Western blot, a band at ~44 kDa is expected. Use reduced and denatured conditions unless optimization suggests otherwise.
• In immunofluorescence/immunocytochemistry, permeabilization is required for intracellular staining. Co-localization with cytoplasmic markers is recommended.
• ELISA applications may require pairing with a matched detection antibody; consider in-solution conditions for antigen capture.
• Since SERPINB13 is skin-specific, include relevant positive controls such as human keratinocyte cell lines (e.g., HaCaT) or skin tissue lysates.
• Cross-reactivity with mouse and rat is reported; validate in your specific model system as reactivity may vary with sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, serving diagnostic manufacturers and research labs from concept to clinic. This anti-SERPINB13 polyclonal antibody is ideal for dermatology and keratinocyte biology research, and can be integrated into assay development for skin-related biomarkers. In addition to this product, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your diagnostic pipeline.